caa a22 4500 445818697 CHVBK 20180317145237.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s00792-011-0385-0 doi (NATIONALLICENCE)springer-10.1007/s00792-011-0385-0 Characterization of Sulfolobus islandicus rod-shaped virus 2 gp19, a single-strand specific endonuclease [Elektronische Daten] [Andrew Gardner, David Prangishvili, William Jack] The hyperthermophilic Sulfolobus islandicus rod-shaped virus 2 (SIRV2) encodes a 25-kDa protein (SIRV2gp19) annotated as a hypothetical protein with sequence homology to the RecB nuclease superfamily. Even though SIRV2gp19 homologs are conserved throughout the rudivirus family and presumably play a role in the viral life cycle, SIRV2gp19 has not been functionally characterized. To define the minimal requirements for activity, SIRV2gp19 was purified and tested under varying conditions. SIRV2gp19 is a single-strand specific endonuclease that requires Mg2+ for activity and is inactive on double-stranded DNA. A conserved aspartic acid in RecB nuclease superfamily Motif II (D89) is also essential for SIRV2gp19 activity and mutation to alanine (D89A) abolishes activity. Therefore, the SIRV2gp19 cleavage mechanism is similar to previously described RecB nucleases. Finally, SIRV2gp19 single-stranded DNA endonuclease activity could play a role in host chromosome degradation during SIRV2 lytic infection. The Author(s), 2011 Archaeal DNA replication nationallicence Biochemical characterization nationallicence Enzyme mechanism nationallicence Molecular biology of Archaea nationallicence Archaeal virus nationallicence Gardner Andrew New England Biolabs, Inc., 240 County Road, 01938, Ipswich, MA, USA aut Prangishvili David Biologie Moléculaire du Gène chez les Extrêmophiles, Institut Pasteur, 25 rue du Docteur Roux, 75724, Paris Cedex 15, France aut Jack William New England Biolabs, Inc., 240 County Road, 01938, Ipswich, MA, USA aut Extremophiles Springer Japan 15/5(2011-09-01), 619-624 1431-0651 15:5<619 2011 15 792 https://doi.org/10.1007/s00792-011-0385-0 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00792-011-0385-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gardner Andrew New England Biolabs, Inc., 240 County Road, 01938, Ipswich, MA, USA aut NATIONALLICENCE 700 1- Prangishvili David Biologie Moléculaire du Gène chez les Extrêmophiles, Institut Pasteur, 25 rue du Docteur Roux, 75724, Paris Cedex 15, France aut NATIONALLICENCE 700 1- Jack William New England Biolabs, Inc., 240 County Road, 01938, Ipswich, MA, USA aut NATIONALLICENCE 773 0- Extremophiles Springer Japan 15/5(2011-09-01), 619-624 1431-0651 15:5<619 2011 15 792 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer