caa a22 4500 445818719 CHVBK 20180317145237.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s00792-011-0390-3 doi (NATIONALLICENCE)springer-10.1007/s00792-011-0390-3 Interactions between P-limitation and different C conditions on the fatty acid composition of an extremophile microalga [Elektronische Daten] [Elly Spijkerman, Alexander Wacker] The extremophilic microalga Chlamydomonas acidophila inhabits very acidic waters (pH 2-3.5), where its growth is often limited by phosphorus (P) or colimited by P and inorganic carbon (CO2). Because this alga is a major food source for predators in acidic habitats, we studied its fatty acid content, which reflects their quality as food, grown under a combination of P-limited and different carbon conditions (either mixotrophically with light+glucose or at high or low CO2, both without glucose). The fatty acid composition largely depended on the cellular P content: stringent P-limited cells had a higher total fatty acid concentration and had a lower percentage of polyunsaturated fatty acids. An additional limitation for CO2 inhibited this decrease, especially reflected in enhanced concentrations of 18:3(9,12,15) and 16:4(3,7,10,13), resulting in cells relatively rich in polyunsaturated fatty acids under colimiting growth conditions. The percentage of polyunsaturated to total fatty acid content was positively related with maximum photosynthesis under all conditions applied. The two factors, P and CO2, thus interact in their effect on the fatty acid composition in C. acidophila, and colimited cells P-limited algae can be considered a superior food source for herbivores because of the high total fatty acid content and relative richness in polyunsaturated fatty acids. Springer, 2011 Acidophilic algae nationallicence Cellular P quota nationallicence Chlamydomonas acidophila nationallicence Chlorophyceae nationallicence Colimitation nationallicence CO2 nationallicence Fatty acid composition nationallicence Food quality nationallicence Glucose nationallicence Mixotrophy nationallicence Photosynthesis nationallicence Phytoplankton nationallicence Phosphorus limitation nationallicence Alpha (α) : Initial slope of the photosynthesis-irradiance curve nationallicence ANCOVA : Analysis of covariance nationallicence Beta (β) : Slope at high irradiances that describes photo-inhibition nationallicence DGDG : Digalactosyldiacylglycerol nationallicence FA : Fatty acid nationallicence I : Actinic light intensity nationallicence K m(CO2) : Half saturation constant for CO2 uptake nationallicence μ : Growth rate nationallicence MUFA : Monounsaturated fatty acid nationallicence Mixotrophy : Growth on glucose and light nationallicence P : Phosphorus nationallicence Pr : Photosynthetic rate nationallicence P max : Maximum photosynthetic rate nationallicence PUFA : Polyunsaturated fatty acid nationallicence Q p : Cellular P quota nationallicence R d : Dark respiration rate nationallicence SFA : Saturated fatty acid nationallicence Spijkerman Elly Department of Ecology and Ecosystem Modelling, University of Potsdam, Am Neuen Palais 10, 14469, Potsdam, Germany aut Wacker Alexander Department of Theoretical Aquatic Ecology, University of Potsdam, Am Neuen Palais 10, 14469, Potsdam, Germany aut Extremophiles Springer Japan 15/5(2011-09-01), 597-609 1431-0651 15:5<597 2011 15 792 https://doi.org/10.1007/s00792-011-0390-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00792-011-0390-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Spijkerman Elly Department of Ecology and Ecosystem Modelling, University of Potsdam, Am Neuen Palais 10, 14469, Potsdam, Germany aut NATIONALLICENCE 700 1- Wacker Alexander Department of Theoretical Aquatic Ecology, University of Potsdam, Am Neuen Palais 10, 14469, Potsdam, Germany aut NATIONALLICENCE 773 0- Extremophiles Springer Japan 15/5(2011-09-01), 597-609 1431-0651 15:5<597 2011 15 792 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer