caa a22 4500 445818735 CHVBK 20180317145237.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s00792-011-0359-2 doi (NATIONALLICENCE)springer-10.1007/s00792-011-0359-2 Acidiferrobacter thiooxydans , gen. nov. sp. nov.; an acidophilic, thermo-tolerant, facultatively anaerobic iron- and sulfur-oxidizer of the family Ectothiorhodospiraceae [Elektronische Daten] [Kevin Hallberg, Sabrina Hedrich, D. Johnson] A comprehensive physiological and phylogenetic characterisation was carried out of "Thiobacillus ferrooxidans” m-1, an acidophilic iron-oxidizing bacterium first described over 25years ago. Phylogenetically, strain m-1 is a gammaproteobacterium, most closely related to alkaliphilic Ectothiorhodospira spp. and only distantly to iron-oxidizing acidithiobacilli. Physiological examination confirmed that strain m-1 can grow autotrophically not only by ferrous iron oxidation but also, in contrast to previous reports, by oxidation of elemental sulfur, sulfide and tetrathionate, using either oxygen or ferric iron as terminal electron acceptor. The bacterium was also found to be thermo-tolerant, growing optimally at 38°C and up to a maximum of 47°C. Growth in liquid media required an external osmotic potential of >2bar, and was optimal at ~5bar, though no growth occurred where the medium osmotic potential was close to that of sea water (~26bar). From this, it was concluded that strain m-1 is a moderate osmophile. Strain m-1 was also shown to be diazotrophic and tolerant of elevated concentrations of many metals typically found in mine-impacted environments. On the basis of these data, m-1 is proposed as the type strain of a new genus and species of bacteria, Acidiferrobacter thiooxydans (DSM 2392, JCM 17358). Springer, 2011 Acidophile nationallicence Diazotroph nationallicence Iron oxidation nationallicence Iron reduction nationallicence Osmophile nationallicence Sulfur oxidation nationallicence Thermo-tolerance nationallicence Hallberg Kevin School of Biological Sciences, College of Natural Sciences, Bangor University, Deiniol Road, LL57 2UW, Bangor, UK aut Hedrich Sabrina School of Biological Sciences, College of Natural Sciences, Bangor University, Deiniol Road, LL57 2UW, Bangor, UK aut Johnson D. School of Biological Sciences, College of Natural Sciences, Bangor University, Deiniol Road, LL57 2UW, Bangor, UK aut Extremophiles Springer-Verlag 15/2(2011-03-01), 271-279 1431-0651 15:2<271 2011 15 792 https://doi.org/10.1007/s00792-011-0359-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00792-011-0359-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hallberg Kevin School of Biological Sciences, College of Natural Sciences, Bangor University, Deiniol Road, LL57 2UW, Bangor, UK aut NATIONALLICENCE 700 1- Hedrich Sabrina School of Biological Sciences, College of Natural Sciences, Bangor University, Deiniol Road, LL57 2UW, Bangor, UK aut NATIONALLICENCE 700 1- Johnson D. School of Biological Sciences, College of Natural Sciences, Bangor University, Deiniol Road, LL57 2UW, Bangor, UK aut NATIONALLICENCE 773 0- Extremophiles Springer-Verlag 15/2(2011-03-01), 271-279 1431-0651 15:2<271 2011 15 792 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer