caa a22 4500 445818816 CHVBK 20180317145237.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s00792-011-0361-8 doi (NATIONALLICENCE)springer-10.1007/s00792-011-0361-8 Fusion of the OsmC domain from esterase EstO confers thermolability to the cold-active xylanase Xyn8 from Pseudoalteromonas arctica [Elektronische Daten] [Skander Elleuche, Henning Piascheck, Garabed Antranikian] The OsmC-region (osmotically induced protein family) of the two-domain esterase EstO from the psychrotolerant bacterium Pseudoalteromonas arctica has been shown to increase thermolability. In an attempt to test if these properties can be conferred to another enzyme, we genetically fused osmC to the 3′-region of the family 8 xylanase encoding gene xyn8 from P. arctica. The chimeric open reading frame xyn8-OsmC was cloned and the chimeric protein was purified after heterologous expression in Escherichia coli. Xyn8 and Xyn8-OsmC showed cold-adapted properties (more than 60% activity at 0°C) using birchwood xylan as the preferred substrate. Maximal catalytic activity is slightly shifted from 15°C (Xyn8) to 20°C for Xyn8-OsmC. Thermostability of Xyn8-OsmC is significantly changed in comparison to wild-type Xyn8. The OsmC-fusion variant showed an apparent decrease in thermostability between 40 and 45°C, while both proteins are highly instable at 50°C. Springer, 2011 Xylanase nationallicence OsmC nationallicence Pseudoalteromonas arctica nationallicence Thermolability nationallicence Gene fusion nationallicence Esterase nationallicence Elleuche Skander Institute of Technical Microbiology, Hamburg University of Technology, Kasernenstr. 12, 21073, Hamburg, Germany aut Piascheck Henning Institute of Technical Microbiology, Hamburg University of Technology, Kasernenstr. 12, 21073, Hamburg, Germany aut Antranikian Garabed Institute of Technical Microbiology, Hamburg University of Technology, Kasernenstr. 12, 21073, Hamburg, Germany aut Extremophiles Springer Japan 15/2(2011-03-01), 311-317 1431-0651 15:2<311 2011 15 792 https://doi.org/10.1007/s00792-011-0361-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00792-011-0361-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Elleuche Skander Institute of Technical Microbiology, Hamburg University of Technology, Kasernenstr. 12, 21073, Hamburg, Germany aut NATIONALLICENCE 700 1- Piascheck Henning Institute of Technical Microbiology, Hamburg University of Technology, Kasernenstr. 12, 21073, Hamburg, Germany aut NATIONALLICENCE 700 1- Antranikian Garabed Institute of Technical Microbiology, Hamburg University of Technology, Kasernenstr. 12, 21073, Hamburg, Germany aut NATIONALLICENCE 773 0- Extremophiles Springer Japan 15/2(2011-03-01), 311-317 1431-0651 15:2<311 2011 15 792 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer