caa a22 4500 445818824 CHVBK 20180317145237.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s00792-011-0360-9 doi (NATIONALLICENCE)springer-10.1007/s00792-011-0360-9 Production of ectoine through a combined process that uses both growing and resting cells of Halomonas salina DSM 5928T [Elektronische Daten] [Ya-jun Lang, Lin Bai, Ya-nan Ren, Ling-hua Zhang, Shinichi Nagata] Using ectoine-excreting strain Halomonas salina DSM 5928T, we developed a new process for high-efficiency production of ectoine, which involved a combined process of batch fermentation by growing cells and production by resting cells. In the first stage, batch fermentation was carried out using growing cells under optimal fermentation conditions. The second stage was the production phase, in which ectoine was synthesized and excreted by phosphate-limited resting cells. Optimal conditions for synthesis and excretion of ectoine during batch fermentation in a 10l fermentor were 0.5moll−1 NaCl and an initial monosodium glutamate concentration of 80gl−1 respectively. The pH was adjusted to 7.0 and the temperature was maintained at 33°C. In phosphate-limited resting cells medium, monosodium glutamate and NaCl concentration was 200gl−1 and 0.5moll−1, respectively, as well as pH was 7.0. The total concentration of ectoine produced was 14.86gl−1, the productivity and yield of ectoine was 7.75gl−1day−1 and 0.14gg−1, respectively, and the percentage of ectoine excreted was 79%. These levels of ectoine production and excretion are the highest reported to date. Springer, 2011 Halomonas salina DSM 5928T nationallicence Ectoine excretion nationallicence Growing cells nationallicence Resting cells nationallicence Ectoine concentration nationallicence Lang Ya-jun Environmental Science and Engineering College, Dalian Maritime University, 1 Linghai Road, 116026, Dalian, People's Republic of China aut Bai Lin Environmental Science and Engineering College, Dalian Maritime University, 1 Linghai Road, 116026, Dalian, People's Republic of China aut Ren Ya-nan Environmental Science and Engineering College, Dalian Maritime University, 1 Linghai Road, 116026, Dalian, People's Republic of China aut Zhang Ling-hua Environmental Science and Engineering College, Dalian Maritime University, 1 Linghai Road, 116026, Dalian, People's Republic of China aut Nagata Shinichi Research Center for Inland Seas, Kobe University, 658-0022, Kobe, Japan aut Extremophiles Springer Japan 15/2(2011-03-01), 303-310 1431-0651 15:2<303 2011 15 792 https://doi.org/10.1007/s00792-011-0360-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00792-011-0360-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lang Ya-jun Environmental Science and Engineering College, Dalian Maritime University, 1 Linghai Road, 116026, Dalian, People's Republic of China aut NATIONALLICENCE 700 1- Bai Lin Environmental Science and Engineering College, Dalian Maritime University, 1 Linghai Road, 116026, Dalian, People's Republic of China aut NATIONALLICENCE 700 1- Ren Ya-nan Environmental Science and Engineering College, Dalian Maritime University, 1 Linghai Road, 116026, Dalian, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Ling-hua Environmental Science and Engineering College, Dalian Maritime University, 1 Linghai Road, 116026, Dalian, People's Republic of China aut NATIONALLICENCE 700 1- Nagata Shinichi Research Center for Inland Seas, Kobe University, 658-0022, Kobe, Japan aut NATIONALLICENCE 773 0- Extremophiles Springer Japan 15/2(2011-03-01), 303-310 1431-0651 15:2<303 2011 15 792 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer