caa a22 4500 445818840 CHVBK 20180317145237.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s00792-011-0357-4 doi (NATIONALLICENCE)springer-10.1007/s00792-011-0357-4 Heterologous expression and characterization of a recombinant thermostable alkylsulfatase ( sdsAP ) [Elektronische Daten] [Mengxian Long, Lingwei Ruan, Fuying Li, Ziniu Yu, Xun Xu] A novel alkylsulfatase gene, sdsAP, was cloned from a newly isolated bacterium Pseudomonas sp. S9. It encoded a protein of 675 amino acids with a calculated molecular mass of 74.9kDa. The protein contained a typical N-terminal signal peptide of 41 amino acid residues, followed by a metallo-β-lactamase like domain at the N-terminus and a SCP-2-like domain at the C-terminus. This domain organization mode suggested that it belonged to the type III sulfatase. The mature alkylsulfatase was overexpressed in Escherichia coli. The optimal temperature and pH of the recombinant SdsAP were 70°C and 9.0, respectively. Notably, at optimal conditions, the purified recombinant SdsAP had a high specific activity of 23.25μmolmin−1mg−1, a K m (app) of 264.3μmol, and a V max (app) of 33.8μmolmin−1mg−1 for SDS. Additionally, it still retained more than 90% activity after incubation at 65°C for 1h, which was much different from other alkylsulfatases reported. The recombinant enzyme hydrolyzed the primary alkyl sulfate such as sodium octyl sulfate and sodium dodecyl sulfate (SDS). It was a Zn2+-containing and Ca2+ activated alkylsulfatase. This is the first report to explore the various characteristics of the heterologous recombinant alkylsulfatase in details. These favorable properties could make SdsAP attractive to be useful in the degradation of SDS-containing waste. Springer, 2011 Alkylsulfatase nationallicence Pseudomonas sp nationallicence Characterization nationallicence Mangrove nationallicence Thermostability nationallicence SDS nationallicence Long Mengxian State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, 430070, Wuhan, Hubei, People's Republic of China aut Ruan Lingwei Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration (SOA), No. 178 Daxue Road, 361005, Xiamen, People's Republic of China aut Li Fuying Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration (SOA), No. 178 Daxue Road, 361005, Xiamen, People's Republic of China aut Yu Ziniu State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, 430070, Wuhan, Hubei, People's Republic of China aut Xu Xun Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration (SOA), No. 178 Daxue Road, 361005, Xiamen, People's Republic of China aut Extremophiles Springer Japan 15/2(2011-03-01), 293-301 1431-0651 15:2<293 2011 15 792 https://doi.org/10.1007/s00792-011-0357-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00792-011-0357-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Long Mengxian State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, 430070, Wuhan, Hubei, People's Republic of China aut NATIONALLICENCE 700 1- Ruan Lingwei Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration (SOA), No. 178 Daxue Road, 361005, Xiamen, People's Republic of China aut NATIONALLICENCE 700 1- Li Fuying Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration (SOA), No. 178 Daxue Road, 361005, Xiamen, People's Republic of China aut NATIONALLICENCE 700 1- Yu Ziniu State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, 430070, Wuhan, Hubei, People's Republic of China aut NATIONALLICENCE 700 1- Xu Xun Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration (SOA), No. 178 Daxue Road, 361005, Xiamen, People's Republic of China aut NATIONALLICENCE 773 0- Extremophiles Springer Japan 15/2(2011-03-01), 293-301 1431-0651 15:2<293 2011 15 792 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer