caa a22 4500 445818859 CHVBK 20180317145237.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s00792-010-0354-z doi (NATIONALLICENCE)springer-10.1007/s00792-010-0354-z Isolation and characterization of a new bacteriophage MMP17 from Meiothermus [Elektronische Daten] [Lianbing Lin, Jian Han, Xiuling Ji, Wei Hong, Li Huang, Yunlin Wei] Thermophiles and their viruses are extraordinarily important because of their roles in processes of evolution, biogeochemistry and ecology. Species of the genus Meiothermus share close relationship with genus Thermus, but no Meiothermus bacteriophage has been reported yet. In this work, a new thermophilic bacteriophage named MMP17 (Meiothermus Myoviridae phage 17) was isolated from a Meiothermus strain and was further characterized. MMP17 was a typical myovirus with an icosahedral head (42nm in diameter) and a tail (120nm in length and 17nm in width). Its DNA was about 33.5-39.5kb in size. MMP17 was very stable at 55-60°C and pH 6-7. According to the one-step growth curve, the latent period and the burst period were 60 and 30min, respectively. An average of 15 phage particles was released from each infected cell. Four major bands were detected in purified virion preparation by SDS-PAGE. As MMP17 was a thermophilic bacteriophage with lower production temperature, its characterization and the relationship between MMP17 and Thermus bacteriophages deserved for further study. Springer, 2011 Thermophile nationallicence Bacteriophages nationallicence Meiothermus nationallicence Myovirus nationallicence Eryuan hot spring nationallicence Lin Lianbing Biotechnology Research Center, Kunming University of Science and Technology, Bailongsi, 650224, Kunming, Yunnan, China aut Han Jian Biotechnology Research Center, Kunming University of Science and Technology, Bailongsi, 650224, Kunming, Yunnan, China aut Ji Xiuling Biotechnology Research Center, Kunming University of Science and Technology, Bailongsi, 650224, Kunming, Yunnan, China aut Hong Wei Biotechnology Research Center, Kunming University of Science and Technology, Bailongsi, 650224, Kunming, Yunnan, China aut Huang Li State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, 100101, Beijing, China aut Wei Yunlin Biotechnology Research Center, Kunming University of Science and Technology, Bailongsi, 650224, Kunming, Yunnan, China aut Extremophiles Springer Japan 15/2(2011-03-01), 253-258 1431-0651 15:2<253 2011 15 792 https://doi.org/10.1007/s00792-010-0354-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00792-010-0354-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lin Lianbing Biotechnology Research Center, Kunming University of Science and Technology, Bailongsi, 650224, Kunming, Yunnan, China aut NATIONALLICENCE 700 1- Han Jian Biotechnology Research Center, Kunming University of Science and Technology, Bailongsi, 650224, Kunming, Yunnan, China aut NATIONALLICENCE 700 1- Ji Xiuling Biotechnology Research Center, Kunming University of Science and Technology, Bailongsi, 650224, Kunming, Yunnan, China aut NATIONALLICENCE 700 1- Hong Wei Biotechnology Research Center, Kunming University of Science and Technology, Bailongsi, 650224, Kunming, Yunnan, China aut NATIONALLICENCE 700 1- Huang Li State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, 100101, Beijing, China aut NATIONALLICENCE 700 1- Wei Yunlin Biotechnology Research Center, Kunming University of Science and Technology, Bailongsi, 650224, Kunming, Yunnan, China aut NATIONALLICENCE 773 0- Extremophiles Springer Japan 15/2(2011-03-01), 253-258 1431-0651 15:2<253 2011 15 792 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer