caa a22 4500 445818883 CHVBK 20180317145237.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s00792-010-0353-0 doi (NATIONALLICENCE)springer-10.1007/s00792-010-0353-0 The bindosome is a structural component of the Sulfolobus solfataricus cell envelope [Elektronische Daten] [Benham Zolghadr, Andreas Klingl, Reinard Rachel, Arnold Driessen, Sonja-Verena Albers] Sugar binding proteins of the thermoacidophile Sulfolobus solfataricus function together with ABC transporters in the uptake of sugars. They are synthesized as precursors with a class III signal peptide that are normally found in archaeal flagellins and bacterial type IV pilins. The functional expression of sugar binding proteins at the cell surface is dependent on the bindosome assembly system (Bas) that is homologous to bacterial type IV pilin assembly systems. The Bas system consists of an assembly ATPase, BasE; a membrane anchoring protein, BasF; and three small class III signal peptide containing proteins BasABC. Expression of BasEF in a S. solfataricus ΔbasEF strain restored the uptake of glucose, while an ATPase mutant of BasE was unable to complement. BasEF was detergent-extracted from S. solfataricus membranes as a stable protein complex. Solute binding proteins can be extracted from the cell surface as two high molecular mass complexes of 600 and 400kDa, wherein the largest complex also contains the main S-layer protein SlaA. Electron microscopic analysis of the cell surface of the wild-type and ΔbasEF strain indicates that the absence of the BasEF complex causes an alteration in cell morphology and the corrugation of the S-layer pattern that is reversed by complementation with the BasEF complex. These results suggest an interaction between the S-layer and the sugar binding proteins that contribute to cell shape. The Author(s), 2011 Cell envelope nationallicence Archaea nationallicence Sulfolobus nationallicence S-layer nationallicence Zolghadr Benham Molecular Biology of Archaea, Max-Planck-Institute for Terrestrial Microbiology, Karl-von-Frisch-Strasse 10, 35043, Marburg, Germany aut Klingl Andreas Centre for Electron Microscopy, Institute for Microbiology, Universität Regensburg, Universitätsstr. 31, 93053, Regensburg, Germany aut Rachel Reinard Centre for Electron Microscopy, Institute for Microbiology, Universität Regensburg, Universitätsstr. 31, 93053, Regensburg, Germany aut Driessen Arnold Department of Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, The Zernike Institute for Advanced Materials, University of Groningen, Kerklaan 30, 9751 NN, Haren, The Netherlands aut Albers Sonja-Verena Molecular Biology of Archaea, Max-Planck-Institute for Terrestrial Microbiology, Karl-von-Frisch-Strasse 10, 35043, Marburg, Germany aut Extremophiles Springer Japan 15/2(2011-03-01), 235-244 1431-0651 15:2<235 2011 15 792 https://doi.org/10.1007/s00792-010-0353-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00792-010-0353-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zolghadr Benham Molecular Biology of Archaea, Max-Planck-Institute for Terrestrial Microbiology, Karl-von-Frisch-Strasse 10, 35043, Marburg, Germany aut NATIONALLICENCE 700 1- Klingl Andreas Centre for Electron Microscopy, Institute for Microbiology, Universität Regensburg, Universitätsstr. 31, 93053, Regensburg, Germany aut NATIONALLICENCE 700 1- Rachel Reinard Centre for Electron Microscopy, Institute for Microbiology, Universität Regensburg, Universitätsstr. 31, 93053, Regensburg, Germany aut NATIONALLICENCE 700 1- Driessen Arnold Department of Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, The Zernike Institute for Advanced Materials, University of Groningen, Kerklaan 30, 9751 NN, Haren, The Netherlands aut NATIONALLICENCE 700 1- Albers Sonja-Verena Molecular Biology of Archaea, Max-Planck-Institute for Terrestrial Microbiology, Karl-von-Frisch-Strasse 10, 35043, Marburg, Germany aut NATIONALLICENCE 773 0- Extremophiles Springer Japan 15/2(2011-03-01), 235-244 1431-0651 15:2<235 2011 15 792 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer