caa a22 4500 445820268 CHVBK 20180317145242.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s00338-010-0687-1 doi (NATIONALLICENCE)springer-10.1007/s00338-010-0687-1 Preservation of corals in salt-saturated DMSO buffer is superior to ethanol for PCR experiments [Elektronische Daten] [M. Gaither, Z. Szabó, M. Crepeau, C. Bird, R. Toonen] Specimen collection is time consuming and expensive, yet few laboratories test preservation methods before setting out on field expeditions. The most common preservation buffer used for coral specimens is >70% EtOH. However, alternatives exist that are less flammable, easier to ship, and are widely used in other taxa. Here, we compare the effects of salt-saturated DMSO (SSD) and EtOH preservation buffers on post-extraction DNA quantity and quality. We found that soft tissue integrity was better maintained and higher quantities of DNA were extracted from EtOH-preserved specimens; however, by all other measures, SSD was a superior preservative to EtOH. Extractions of SSD-preserved specimens resulted in higher molecular weight DNA, higher PCR success, and more efficient amplification than specimens preserved in EtOH. Our results show that SSD is generally a superior preservative to EtOH for specimens destined for PCR studies, but species-specific differences indicate that preservation comparisons should be undertaken before collection and storage of samples. Springer-Verlag, 2010 Preservation methods nationallicence Real-time quantitative PCR nationallicence qPCR nationallicence Scleractinian coral nationallicence Storage solutions nationallicence Gaither M. Hawai‘i Institute of Marine Biology, P.O. Box 1346, 96744, Kāne‘ohe, Hawai‘i, USA aut Szabó Z. Hawai‘i Institute of Marine Biology, P.O. Box 1346, 96744, Kāne‘ohe, Hawai‘i, USA aut Crepeau M. Genome and Biomedical Sciences Facility, University of California, Davis, 451 Health Sciences Drive, 95616, Davis, CA, USA aut Bird C. Hawai‘i Institute of Marine Biology, P.O. Box 1346, 96744, Kāne‘ohe, Hawai‘i, USA aut Toonen R. Hawai‘i Institute of Marine Biology, P.O. Box 1346, 96744, Kāne‘ohe, Hawai‘i, USA aut Coral Reefs Springer-Verlag 30/2(2011-06-01), 329-333 0722-4028 30:2<329 2011 30 338 https://doi.org/10.1007/s00338-010-0687-1 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00338-010-0687-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gaither M. Hawai‘i Institute of Marine Biology, P.O. Box 1346, 96744, Kāne‘ohe, Hawai‘i, USA aut NATIONALLICENCE 700 1- Szabó Z. Hawai‘i Institute of Marine Biology, P.O. Box 1346, 96744, Kāne‘ohe, Hawai‘i, USA aut NATIONALLICENCE 700 1- Crepeau M. Genome and Biomedical Sciences Facility, University of California, Davis, 451 Health Sciences Drive, 95616, Davis, CA, USA aut NATIONALLICENCE 700 1- Bird C. Hawai‘i Institute of Marine Biology, P.O. Box 1346, 96744, Kāne‘ohe, Hawai‘i, USA aut NATIONALLICENCE 700 1- Toonen R. Hawai‘i Institute of Marine Biology, P.O. Box 1346, 96744, Kāne‘ohe, Hawai‘i, USA aut NATIONALLICENCE 773 0- Coral Reefs Springer-Verlag 30/2(2011-06-01), 329-333 0722-4028 30:2<329 2011 30 338 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer