caa a22 4500 445820802 CHVBK 20180317145243.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s00239-011-9464-1 doi (NATIONALLICENCE)springer-10.1007/s00239-011-9464-1 A Chimeric Chromosome in the Ciliate Oxytricha Resulting from Duplication [Elektronische Daten] [Yi Zhou, Helmae Wubneh, Clayton Schwarz, Laura Landweber] In a process similar to exon splicing, ciliates use DNA splicing to produce a new somatic macronuclear genome from their germline micronuclear genome after sexual reproduction. This extra layer of DNA rearrangement permits novel mechanisms to create genetic complexity during both evolution and development. Here we describe a chimeric macronuclear chromosome in Oxytricha trifallax constructed from two smaller macronuclear chromosomes. To determine how the chimera was generated, we cloned and sequenced the corresponding germline loci. The chimera derives from a novel locus in the micronucleus that arose by partial duplication of the loci for the two smaller chromosomes. This suggests that an exon shuffling-like process, which we call MDS shuffling, enables ciliates to generate novel genetic material and gene products using different combinations of genomic DNA segments. Springer Science+Business Media, LLC, 2011 Ciliate nationallicence Duplication nationallicence Gene shuffling nationallicence Chimeric chromosome nationallicence Micronucleus nationallicence Oxytricha nationallicence MAC : Macronucleus nationallicence MIC : Micronucleus nationallicence MDS : Macronuclear-destined sequence nationallicence IES : Internal-eliminated sequence nationallicence Zhou Yi Department of Ecology and Evolutionary Biology, Princeton University, 08544, Princeton, NJ, USA aut Wubneh Helmae Department of Ecology and Evolutionary Biology, Princeton University, 08544, Princeton, NJ, USA aut Schwarz Clayton Department of Ecology and Evolutionary Biology, Princeton University, 08544, Princeton, NJ, USA aut Landweber Laura Department of Ecology and Evolutionary Biology, Princeton University, 08544, Princeton, NJ, USA aut Journal of Molecular Evolution Springer-Verlag 73/3-4(2011-10-01), 70-73 0022-2844 73:3-4<70 2011 73 239 https://doi.org/10.1007/s00239-011-9464-1 text/html Onlinezugriff via DOI 1 review-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00239-011-9464-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhou Yi Department of Ecology and Evolutionary Biology, Princeton University, 08544, Princeton, NJ, USA aut NATIONALLICENCE 700 1- Wubneh Helmae Department of Ecology and Evolutionary Biology, Princeton University, 08544, Princeton, NJ, USA aut NATIONALLICENCE 700 1- Schwarz Clayton Department of Ecology and Evolutionary Biology, Princeton University, 08544, Princeton, NJ, USA aut NATIONALLICENCE 700 1- Landweber Laura Department of Ecology and Evolutionary Biology, Princeton University, 08544, Princeton, NJ, USA aut NATIONALLICENCE 773 0- Journal of Molecular Evolution Springer-Verlag 73/3-4(2011-10-01), 70-73 0022-2844 73:3-4<70 2011 73 239 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer