caa a22 4500 445820810 CHVBK 20180317145243.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s00239-011-9458-z doi (NATIONALLICENCE)springer-10.1007/s00239-011-9458-z Selection and constraint on regulatory elements in Drosophila simulans [Elektronische Daten] [Tami Cruickshank, Phil Nista] We utilized available Drosophila simulans molecular population genomic data to characterize sequence polymorphism in noncoding regulatory regions and their corresponding transcribed target genes or interacting transcription factors. We highlight two properties of regulatory evolution. First, we find that sequence divergence between D. melanogaster and D. simulans is greater at regulatory sites than expected from levels of intraspecific polymorphism, suggestive of positive selection. Second, we compared variation in cis-regulatory regions to that of the transcription factors and transcribed target genes with which they are associated. We find a correlation in polymorphism between cis-regulatory regions and the transcription factors which they bind, but not between cis-regulatory regions and the physically neighboring target genes which they directly regulate. This result indicates that the evolution of cis-regulatory elements is constrained by the interactions among transcription factors and regulatory regions. More broadly, these findings support the general importance of noncoding regulatory DNA in evolution. Springer Science+Business Media, LLC, 2011 Gene regulation nationallicence Regulatory networks nationallicence Drosophila nationallicence Development nationallicence Molecular evolution nationallicence Cruickshank Tami Department of Biology, Indiana University, 1001 E. 3rd St, Bloomington, IN, USA aut Nista Phil Department of Biology, Indiana University, 1001 E. 3rd St, Bloomington, IN, USA aut Journal of Molecular Evolution Springer-Verlag 73/3-4(2011-10-01), 94-100 0022-2844 73:3-4<94 2011 73 239 https://doi.org/10.1007/s00239-011-9458-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00239-011-9458-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cruickshank Tami Department of Biology, Indiana University, 1001 E. 3rd St, Bloomington, IN, USA aut NATIONALLICENCE 700 1- Nista Phil Department of Biology, Indiana University, 1001 E. 3rd St, Bloomington, IN, USA aut NATIONALLICENCE 773 0- Journal of Molecular Evolution Springer-Verlag 73/3-4(2011-10-01), 94-100 0022-2844 73:3-4<94 2011 73 239 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer