caa a22 4500 445820853 CHVBK 20180317145243.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s00239-011-9460-5 doi (NATIONALLICENCE)springer-10.1007/s00239-011-9460-5 Prestin Shows Divergent Evolution Between Constant Frequency Echolocating Bats [Elektronische Daten] [Bin Shen, Rafael Avila-Flores, Yang Liu, Stephen Rossiter, Shuyi Zhang] The gene Prestin encodes a motor protein that is thought to confer the high-frequency sensitivity and selectivity that characterizes the mammalian auditory system. Recent research shows that the Prestin gene has undergone a burst of positive selection on the ancestral branch of the Old World horseshoe and leaf-nosed bats (Rhinolophidae and Hipposideridae, respectively), and also on the branch leading to echolocating cetaceans. Moreover, these two groups share a large number of convergent amino acid sequence replacements. Horseshoe and leaf-nosed bats exhibit narrowband echolocation, in which the emitted calls are based on the second harmonic of a predominantly constant frequency (CF) component, the frequency of which is also over-represented in the cochlea. This highly specialized form of echolocation has also evolved independently in the neotropical Parnell's mustached bat (Pteronotus parnellii). To test whether the convergent evolution of CF echolocation between lineages has arisen from common changes in the Prestin gene, we sequenced the Prestin coding region (~2,212bp, >99% coverage) in P. parnellii and several related species that use broadband echolocation calls. Our reconstructed Prestin gene tree and amino acid tree showed that P. parnellii did not group together with Old World horseshoe and leaf-nosed bats, but rather clustered within its true sister species. Comparisons of sequences confirmed that P. parnellii shared most amino acid changes with its congeners, and we found no evidence of positive selection in the branch leading to the genus of Pteronotus. Our result suggests that the adaptive changes seen in Prestin in horseshoe and leaf-nosed bats are not necessary for CF echolocation in P. parnellii. Springer Science+Business Media, LLC, 2011 Narrowband echolocation nationallicence Pteronotus nationallicence Horseshoe bats nationallicence Hearing nationallicence Convergent evolution nationallicence Shen Bin Institute of Molecular Ecology and Evolution, Institutes for Advanced Interdisciplinary Research, East China Normal University, 200062, Shanghai, China aut Avila-Flores Rafael Instituto de Ecología, Universidad Nacional Autónoma de México, Circuito Exterior s/n, Ciudad Universitaria, 04510, Mexico, DF, Mexico aut Liu Yang Institute of Molecular Ecology and Evolution, Institutes for Advanced Interdisciplinary Research, East China Normal University, 200062, Shanghai, China aut Rossiter Stephen School of Biological and Chemical Sciences, Queen Mary University of London, E1 4NS, London, UK aut Zhang Shuyi Institute of Molecular Ecology and Evolution, Institutes for Advanced Interdisciplinary Research, East China Normal University, 200062, Shanghai, China aut Journal of Molecular Evolution Springer-Verlag 73/3-4(2011-10-01), 109-115 0022-2844 73:3-4<109 2011 73 239 https://doi.org/10.1007/s00239-011-9460-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00239-011-9460-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Shen Bin Institute of Molecular Ecology and Evolution, Institutes for Advanced Interdisciplinary Research, East China Normal University, 200062, Shanghai, China aut NATIONALLICENCE 700 1- Avila-Flores Rafael Instituto de Ecología, Universidad Nacional Autónoma de México, Circuito Exterior s/n, Ciudad Universitaria, 04510, Mexico, DF, Mexico aut NATIONALLICENCE 700 1- Liu Yang Institute of Molecular Ecology and Evolution, Institutes for Advanced Interdisciplinary Research, East China Normal University, 200062, Shanghai, China aut NATIONALLICENCE 700 1- Rossiter Stephen School of Biological and Chemical Sciences, Queen Mary University of London, E1 4NS, London, UK aut NATIONALLICENCE 700 1- Zhang Shuyi Institute of Molecular Ecology and Evolution, Institutes for Advanced Interdisciplinary Research, East China Normal University, 200062, Shanghai, China aut NATIONALLICENCE 773 0- Journal of Molecular Evolution Springer-Verlag 73/3-4(2011-10-01), 109-115 0022-2844 73:3-4<109 2011 73 239 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer