caa a22 4500 44582087X CHVBK 20180317145243.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s00239-011-9471-2 doi (NATIONALLICENCE)springer-10.1007/s00239-011-9471-2 Self-Replication Reactions Dependent on Tertiary Interaction Motifs in an RNA Ligase Ribozyme [Elektronische Daten] [Rei Ohmori, Hirohide Saito, Yoshiya Ikawa, Yoshihiko Fujita, Tan Inoue] RNA can function both as an informational molecule and as a catalyst in living organisms. This duality is the premise of the RNA world hypothesis. However, one flaw in the hypothesis that RNA was the most essential molecule in primitive life is that no RNA self-replicating system has been found in nature. To verify whether RNA has the potential for self-replication, we constructed a new RNA self-assembling ribozyme that could have conducted an evolvable RNA self-replication reaction. The artificially designed, in vitro selected ligase ribozyme was employed as a prototype for a self-assembling ribozyme. The ribozyme is composed of two RNA fragments (form R1·Z1) that recognize another R1·Z1 molecule as their substrate and perform the high turnover ligation reaction via two RNA tertiary interaction motifs. Furthermore, the substrate recognition of R1·Z1 is tolerant of mutations, generating diversity in the corresponding RNA self-replicating network. Thus, we propose that our system implies the significance of RNA tertiary motifs in the early RNA molecular evolution of the RNA world. Springer Science+Business Media, LLC, 2011 RNA world nationallicence Self-replication nationallicence Ribozyme nationallicence The origin of life nationallicence RNA tertiary interaction motifs nationallicence Primitive RNA evolution nationallicence GNRA loop-receptor nationallicence Ohmori Rei Laboratory of Gene Biodynamics, Graduate School of Biostudies, Kyoto University, Oiwake-cho, Kitashirakawa, 606-8502, Sakyo-ku, Kyoto, Japan aut Saito Hirohide The Hakubi Center, Kyoto University, Oiwake-cho, Kitashirakawa, 606-8502, Sakyo-ku, Kyoto, Japan aut Ikawa Yoshiya Department of Chemistry and Biochemistry, Graduate School of Engineering, Kyushu University, Moto-oka 744, 819-0395, Nishi-ku, Fukuoka, Japan aut Fujita Yoshihiko Laboratory of Gene Biodynamics, Graduate School of Biostudies, Kyoto University, Oiwake-cho, Kitashirakawa, 606-8502, Sakyo-ku, Kyoto, Japan aut Inoue Tan Laboratory of Gene Biodynamics, Graduate School of Biostudies, Kyoto University, Oiwake-cho, Kitashirakawa, 606-8502, Sakyo-ku, Kyoto, Japan aut Journal of Molecular Evolution Springer-Verlag 73/3-4(2011-10-01), 221-229 0022-2844 73:3-4<221 2011 73 239 https://doi.org/10.1007/s00239-011-9471-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00239-011-9471-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ohmori Rei Laboratory of Gene Biodynamics, Graduate School of Biostudies, Kyoto University, Oiwake-cho, Kitashirakawa, 606-8502, Sakyo-ku, Kyoto, Japan aut NATIONALLICENCE 700 1- Saito Hirohide The Hakubi Center, Kyoto University, Oiwake-cho, Kitashirakawa, 606-8502, Sakyo-ku, Kyoto, Japan aut NATIONALLICENCE 700 1- Ikawa Yoshiya Department of Chemistry and Biochemistry, Graduate School of Engineering, Kyushu University, Moto-oka 744, 819-0395, Nishi-ku, Fukuoka, Japan aut NATIONALLICENCE 700 1- Fujita Yoshihiko Laboratory of Gene Biodynamics, Graduate School of Biostudies, Kyoto University, Oiwake-cho, Kitashirakawa, 606-8502, Sakyo-ku, Kyoto, Japan aut NATIONALLICENCE 700 1- Inoue Tan Laboratory of Gene Biodynamics, Graduate School of Biostudies, Kyoto University, Oiwake-cho, Kitashirakawa, 606-8502, Sakyo-ku, Kyoto, Japan aut NATIONALLICENCE 773 0- Journal of Molecular Evolution Springer-Verlag 73/3-4(2011-10-01), 221-229 0022-2844 73:3-4<221 2011 73 239 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer