caa a22 4500 44582090X CHVBK 20180317145243.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s00239-011-9462-3 doi (NATIONALLICENCE)springer-10.1007/s00239-011-9462-3 Phylogenetic and Preliminary Phenotypic Analysis of Yeast PAQR Receptors: Potential Antifungal Targets [Elektronische Daten] [Nancy Villa, Patricia Moussatche, Stephen Chamberlin, Anuj Kumar, Thomas Lyons] Proteins belonging to the Progestin and AdipoQ Receptor (PAQR) superfamily of membrane bound receptors are ubiquitously found in fungi. Nearly, all fungi possess two evolutionarily distinct paralogs of PAQR protein, which we have called the PQRA and PQRB subtypes. In the model fungus Saccharomyces cerevisiae, these subtypes are represented by the Izh2p and Izh3p proteins, respectively. S. cerevisiae also possesses two additional PQRA-type receptors called Izh1p and Izh4p that are restricted to other species within the "Saccharomyces complex”. Izh2p has been the subject of several recent investigations and is of particular interest because it regulates fungal growth in response to proteins produced by plants and, as such, represents a new paradigm for interspecies communication. We demonstrate that IZH2 and IZH3 gene dosage affects resistance to polyene antifungal drugs. Moreover, we provide additional evidence that Izh2p and Izh3p negatively regulate fungal filamentation. These data suggest that agonists of these receptors might make antifungal therapeutics, either by inhibiting fungal development or by sensitizing fungi to the toxic effects of current antifungal therapies. This is particularly relevant for pathogenic fungi such as Candida glabrata that are closely related to S. cerevisiae and contain the same complement of PAQR receptors. The Author(s), 2011 PAQR nationallicence Osmotin nationallicence Fungi nationallicence Saccharomyces cerevisiae nationallicence Antifungal nationallicence Polyene nationallicence Filamentation nationallicence Phylogeny nationallicence Progesterone nationallicence Adiponectin nationallicence Villa Nancy Department of Medicinal Chemistry, College of Pharmacy, University of Florida, P.O. Box 100485, 32610-0485, Gainesville, FL, USA aut Moussatche Patricia Foundation for Applied Molecular Evolution, P.O. Box 13174, 32604, Gainesville, FL, USA aut Chamberlin Stephen Foundation for Applied Molecular Evolution, P.O. Box 13174, 32604, Gainesville, FL, USA aut Kumar Anuj Departments of Molecular, Cellular, and Developmental Biology, Life Sciences Institute, University of Michigan, 210 Washtenaw Avenue, 48109-2216, Ann Arbor, MI, USA aut Lyons Thomas Foundation for Applied Molecular Evolution, P.O. Box 13174, 32604, Gainesville, FL, USA aut Journal of Molecular Evolution Springer-Verlag 73/3-4(2011-10-01), 134-152 0022-2844 73:3-4<134 2011 73 239 https://doi.org/10.1007/s00239-011-9462-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00239-011-9462-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Villa Nancy Department of Medicinal Chemistry, College of Pharmacy, University of Florida, P.O. Box 100485, 32610-0485, Gainesville, FL, USA aut NATIONALLICENCE 700 1- Moussatche Patricia Foundation for Applied Molecular Evolution, P.O. Box 13174, 32604, Gainesville, FL, USA aut NATIONALLICENCE 700 1- Chamberlin Stephen Foundation for Applied Molecular Evolution, P.O. Box 13174, 32604, Gainesville, FL, USA aut NATIONALLICENCE 700 1- Kumar Anuj Departments of Molecular, Cellular, and Developmental Biology, Life Sciences Institute, University of Michigan, 210 Washtenaw Avenue, 48109-2216, Ann Arbor, MI, USA aut NATIONALLICENCE 700 1- Lyons Thomas Foundation for Applied Molecular Evolution, P.O. Box 13174, 32604, Gainesville, FL, USA aut NATIONALLICENCE 773 0- Journal of Molecular Evolution Springer-Verlag 73/3-4(2011-10-01), 134-152 0022-2844 73:3-4<134 2011 73 239 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer