caa a22 4500 445820926 CHVBK 20180317145243.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s00239-011-9459-y doi (NATIONALLICENCE)springer-10.1007/s00239-011-9459-y A Conserved Regulatory Element in the Mammalian β-Globin Promoters [Elektronische Daten] [Ryoiti Kiyama, Yuko Wada-Kiyama] We provide here evidence for a conserved regulatory element for transcription of the β-family globin genes based on a comparative study of 32 genes from 16 mammals. The element is characterized by the appearance of AA or TT dinucleotides in the A+T-rich region located 200-400bp upstream of the cap sites. G-tracts 3-5 nucleotides long exist between the A+T-rich region and the conserved transcription factor binding sites (GATA-1 site and the CACCC, CCAAT, and ATA boxes) apparently dividing the regions. The average periodicity of AA or TT dinucleotides in the region from a total of 18 β-family globin genes from four species was approximately 10bp, suggesting that the DNA in these regions shows right-handed superhelicity. The proposed biological function of this element is to adjust the spatial positions for the first interaction of the transcription factor(s) which can recognize specific DNA sequences in the presence of packed chromatin. Springer Science+Business Media, LLC, 2011 Globin gene nationallicence Promoter nationallicence Transcription nationallicence Conserved sequence nationallicence Curved DNA nationallicence Kiyama Ryoiti Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology, 1-1-1 Higashi, 305-8566, Tsukuba, Ibaraki, Japan aut Wada-Kiyama Yuko Department of Physiology, Nippon Medical School, Sendagi, Bunkyo-ku, 113-8602, Tokyo, Japan aut Journal of Molecular Evolution Springer-Verlag 73/3-4(2011-10-01), 101-108 0022-2844 73:3-4<101 2011 73 239 https://doi.org/10.1007/s00239-011-9459-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00239-011-9459-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kiyama Ryoiti Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology, 1-1-1 Higashi, 305-8566, Tsukuba, Ibaraki, Japan aut NATIONALLICENCE 700 1- Wada-Kiyama Yuko Department of Physiology, Nippon Medical School, Sendagi, Bunkyo-ku, 113-8602, Tokyo, Japan aut NATIONALLICENCE 773 0- Journal of Molecular Evolution Springer-Verlag 73/3-4(2011-10-01), 101-108 0022-2844 73:3-4<101 2011 73 239 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer