caa a22 4500 445820942 CHVBK 20180317145244.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s00239-011-9479-7 doi (NATIONALLICENCE)springer-10.1007/s00239-011-9479-7 Two Polymorphic Residues Account for the Differences in DNA Binding and Transcriptional Activation by NF-κB Proteins Encoded by Naturally Occurring Alleles in Nematostella vectensis [Elektronische Daten] [Francis Wolenski, Sushil Chandani, Derek Stefanik, Ning Jiang, Emma Chu, John Finnerty, Thomas Gilmore] The NF-κB family of transcription factors is activated in response to many environmental and biological stresses, and plays a key role in innate immunity across a broad evolutionary expanse of animals. A simple NF-κB pathway is present in the sea anemone Nematostella vectensis, an important model organism in the phylum Cnidaria. Nematostella has previously been shown to have two naturally occurring NF-κB alleles (Nv-NF-κB-C and Nv-NF-κB-S) that encode proteins with different DNA-binding and transactivation abilities. We show here that polymorphic residues 67 (Cys vs. Ser) and 269 (Ala vs. Glu) play complementary roles in determining the DNA-binding activity of the NF-κB proteins encoded by these two alleles and that residue 67 is primarily responsible for the difference in their transactivation ability. Phylogenetic analysis indicates that Nv-NF-κB-S is the derived allele, consistent with its restricted geographic distribution. These results define polymorphic residues that are important for the DNA-binding and transactivating activities of two naturally occurring variants of Nv-NF-κB. The implications for the appearance of the two Nv-NF-κB alleles in natural populations of sea anemones are discussed. Springer Science+Business Media, LLC, 2011 NF-κB nationallicence Nematostella nationallicence Polymorphism nationallicence Evolution nationallicence DNA binding nationallicence Transactivation nationallicence Wolenski Francis Department of Biology, Boston University, 5 Cummington Street, 02215, Boston, MA, USA aut Chandani Sushil Department of Biology, Boston University, 5 Cummington Street, 02215, Boston, MA, USA aut Stefanik Derek Department of Biology, Boston University, 5 Cummington Street, 02215, Boston, MA, USA aut Jiang Ning Department of Biology, Boston University, 5 Cummington Street, 02215, Boston, MA, USA aut Chu Emma Department of Biology, Boston University, 5 Cummington Street, 02215, Boston, MA, USA aut Finnerty John Department of Biology, Boston University, 5 Cummington Street, 02215, Boston, MA, USA aut Gilmore Thomas Department of Biology, Boston University, 5 Cummington Street, 02215, Boston, MA, USA aut Journal of Molecular Evolution Springer-Verlag 73/5-6(2011-12-01), 325-336 0022-2844 73:5-6<325 2011 73 239 https://doi.org/10.1007/s00239-011-9479-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00239-011-9479-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wolenski Francis Department of Biology, Boston University, 5 Cummington Street, 02215, Boston, MA, USA aut NATIONALLICENCE 700 1- Chandani Sushil Department of Biology, Boston University, 5 Cummington Street, 02215, Boston, MA, USA aut NATIONALLICENCE 700 1- Stefanik Derek Department of Biology, Boston University, 5 Cummington Street, 02215, Boston, MA, USA aut NATIONALLICENCE 700 1- Jiang Ning Department of Biology, Boston University, 5 Cummington Street, 02215, Boston, MA, USA aut NATIONALLICENCE 700 1- Chu Emma Department of Biology, Boston University, 5 Cummington Street, 02215, Boston, MA, USA aut NATIONALLICENCE 700 1- Finnerty John Department of Biology, Boston University, 5 Cummington Street, 02215, Boston, MA, USA aut NATIONALLICENCE 700 1- Gilmore Thomas Department of Biology, Boston University, 5 Cummington Street, 02215, Boston, MA, USA aut NATIONALLICENCE 773 0- Journal of Molecular Evolution Springer-Verlag 73/5-6(2011-12-01), 325-336 0022-2844 73:5-6<325 2011 73 239 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer