caa a22 4500 445820977 CHVBK 20180317145244.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s00239-011-9468-x doi (NATIONALLICENCE)springer-10.1007/s00239-011-9468-x Heterogeneous Rates of Molecular Evolution Among Cryptic Species of the Ciliate Morphospecies Chilodonella uncinata [Elektronische Daten] [Laura Katz, Jennifer DeBerardinis, Meaghan Hall, Alexandra Kovner, Micah Dunthorn, Spencer Muse] While molecular analyses have provided insight into the phylogeny of ciliates, the few studies assessing intraspecific variation have largely relied on just a single locus [e.g., nuclear small subunit rDNA (nSSU-rDNA) or mitochondrial cytochrome oxidase I]. In this study, we characterize the diversity of several nuclear protein-coding genes plus both nSSU-rDNA and mitochondrial small subunit rDNA (mtSSU-rDNA) of five isolates of the ciliate morphospecies Chilodonella uncinata. Although these isolates have nearly identical nSSU-rDNA sequences, they differ by up to 8.0% in mtSSU-rDNA. Comparative analyses of all loci, including β-tubulin paralogs, indicate a lack of recombination between strains, demonstrating that the morphospecies C. uncinata consists of multiple cryptic species. Further, there is considerable variation in substitution rates among loci as some protein-coding domains are nearly identical between isolates, while others differ by up to 13.2% at the amino acid level. Combining insights on macronuclear variation among isolates, the focus of this study, with published data from the micronucleus of two of these isolates, indicates that C. uncinata lineages are able to maintain both highly divergent and highly conserved genes within a rapidly evolving germline genome. Springer Science+Business Media, LLC, 2012 Cryptic species nationallicence Molecular evolution nationallicence Genome architecture nationallicence Ciliate nationallicence Katz Laura Department of Biological Sciences, Smith College, 01063, Northampton, MA, USA aut DeBerardinis Jennifer Department of Biological Sciences, Smith College, 01063, Northampton, MA, USA aut Hall Meaghan Department of Biological Sciences, Smith College, 01063, Northampton, MA, USA aut Kovner Alexandra Department of Biological Sciences, Smith College, 01063, Northampton, MA, USA aut Dunthorn Micah Program in Organismic and Evolutionary Biology, UMass-Amherst, 01003, Amherst, MA, USA aut Muse Spencer Department of Statistics, North Carolina State University, 27695-7566, Raleigh, NC, USA aut Journal of Molecular Evolution Springer-Verlag 73/5-6(2011-12-01), 266-272 0022-2844 73:5-6<266 2011 73 239 https://doi.org/10.1007/s00239-011-9468-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00239-011-9468-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Katz Laura Department of Biological Sciences, Smith College, 01063, Northampton, MA, USA aut NATIONALLICENCE 700 1- DeBerardinis Jennifer Department of Biological Sciences, Smith College, 01063, Northampton, MA, USA aut NATIONALLICENCE 700 1- Hall Meaghan Department of Biological Sciences, Smith College, 01063, Northampton, MA, USA aut NATIONALLICENCE 700 1- Kovner Alexandra Department of Biological Sciences, Smith College, 01063, Northampton, MA, USA aut NATIONALLICENCE 700 1- Dunthorn Micah Program in Organismic and Evolutionary Biology, UMass-Amherst, 01003, Amherst, MA, USA aut NATIONALLICENCE 700 1- Muse Spencer Department of Statistics, North Carolina State University, 27695-7566, Raleigh, NC, USA aut NATIONALLICENCE 773 0- Journal of Molecular Evolution Springer-Verlag 73/5-6(2011-12-01), 266-272 0022-2844 73:5-6<266 2011 73 239 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer