caa a22 4500 445820985 CHVBK 20180317145244.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s00239-012-9484-5 doi (NATIONALLICENCE)springer-10.1007/s00239-012-9484-5 Molecular Evolution of Miraculin-Like Proteins in Soybean Kunitz Super-Family [Elektronische Daten] [Purushotham Selvakumar, Deepankar Gahloth, Prabhat Tomar, Nidhi Sharma, Ashwani Sharma] Miraculin-like proteins (MLPs) belong to soybean Kunitz super-family and have been characterized from many plant families like Rutaceae, Solanaceae, Rubiaceae, etc. Many of them possess trypsin inhibitory activity and are involved in plant defense. MLPs exhibit significant sequence identity (~30-95%) to native miraculin protein, also belonging to Kunitz super-family compared with a typical Kunitz family member (~30%). The sequence and structure-function comparison of MLPs with that of a classical Kunitz inhibitor have demonstrated that MLPs have evolved to form a distinct group within Kunitz super-family. Sequence analysis of new genes along with available MLP sequences in the literature revealed three major groups for these proteins. A significant feature of Rutaceae MLP type 2 sequences is the presence of phosphorylation motif. Subtle changes are seen in putative reactive loop residues among different MLPs suggesting altered specificities to specific proteases. In phylogenetic analysis, Rutaceae MLP type 1 and type 2 proteins clustered together on separate branches, whereas native miraculin along with other MLPs formed distinct clusters. Site-specific positive Darwinian selection was observed at many sites in both the groups of Rutaceae MLP sequences with most of the residues undergoing positive selection located in loop regions. The results demonstrate the sequence and thereby the structure-function divergence of MLPs as a distinct group within soybean Kunitz super-family due to biotic and abiotic stresses of local environment. Springer Science+Business Media, LLC, 2012 Rutaceae nationallicence Kunitz super-family nationallicence Miraculin-like proteins nationallicence Sequence analysis nationallicence Evolutionary relationships nationallicence Positive selection nationallicence Selvakumar Purushotham Department of Biotechnology, Indian Institute of Technology Roorkee, 247 667, Roorkee, India aut Gahloth Deepankar Department of Biotechnology, Indian Institute of Technology Roorkee, 247 667, Roorkee, India aut Tomar Prabhat Department of Biotechnology, Indian Institute of Technology Roorkee, 247 667, Roorkee, India aut Sharma Nidhi Department of Biotechnology, Indian Institute of Technology Roorkee, 247 667, Roorkee, India aut Sharma Ashwani Department of Biotechnology, Indian Institute of Technology Roorkee, 247 667, Roorkee, India aut Journal of Molecular Evolution Springer-Verlag 73/5-6(2011-12-01), 369-379 0022-2844 73:5-6<369 2011 73 239 https://doi.org/10.1007/s00239-012-9484-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00239-012-9484-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Selvakumar Purushotham Department of Biotechnology, Indian Institute of Technology Roorkee, 247 667, Roorkee, India aut NATIONALLICENCE 700 1- Gahloth Deepankar Department of Biotechnology, Indian Institute of Technology Roorkee, 247 667, Roorkee, India aut NATIONALLICENCE 700 1- Tomar Prabhat Department of Biotechnology, Indian Institute of Technology Roorkee, 247 667, Roorkee, India aut NATIONALLICENCE 700 1- Sharma Nidhi Department of Biotechnology, Indian Institute of Technology Roorkee, 247 667, Roorkee, India aut NATIONALLICENCE 700 1- Sharma Ashwani Department of Biotechnology, Indian Institute of Technology Roorkee, 247 667, Roorkee, India aut NATIONALLICENCE 773 0- Journal of Molecular Evolution Springer-Verlag 73/5-6(2011-12-01), 369-379 0022-2844 73:5-6<369 2011 73 239 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer