caa a22 4500 445821035 CHVBK 20180317145244.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s00239-011-9478-8 doi (NATIONALLICENCE)springer-10.1007/s00239-011-9478-8 Misexpression of Testicular MicroRNA in Sterile Xenopus Hybrids Points to Tetrapod-Specific MicroRNAs Associated with Male Fertility [Elektronische Daten] [Mercedita Madison-Villar, Pawel Michalak] Spermatogenesis is one of the most complex biological processes undergone by any organism, making it susceptible to perturbations that result in male sterility. Research has demonstrated that mutant phenotypes can be obtained from the disruption of epigenetic modifications, which are commonly microRNA guided. Employing the Xenopus system, whereby homogametic interspecies males are always sterile, thus violating Haldane's Rule, we deep-sequenced testes-specific small-RNAs to identify microRNAs most frequently misexpressed between sterile hybrids and their fertile parental taxa. Using these data, we cross-referenced our expression information with previously published mouse (Mus musculus) data and identified a subset of seven microRNAs common to both (miR-338, miR-222, miR-18, miR-30, miR-10, miR-196, and miR-365). We propose that these microRNAs are likely critical for spermatogenesis in all tetrapods, having retained testicular expression across ~350million years of evolution (Amphibian-Mammal split). Gene targets of six of these microRNAs are known, and all the six associate with zinc and zinc finger proteins (both previously found critical in male fertility), and three with Hox genes (some of which have also previously been deemed critical for testicular development and male fertility). Expression information for these targets revealed that all those associated with zinc have previously been found to express in mammalian testes. One Hox target has known mammalian testicular expression, two have close relatives with known mammalian testicular expression, and two more are associated with proteins known to have mammalian testicular expression. In addition, miR-222 has prior association with spermatogenesis, and miR-30 has been found to be abundantly expressed in both mouse and human testes. Springer Science+Business Media, LLC, 2011 Xenopus nationallicence Hybrid nationallicence Male sterility nationallicence MicroRNA nationallicence Tetrapod nationallicence Faster male evolution nationallicence Madison-Villar Mercedita UT Arlington, Arlington, TX, USA aut Michalak Pawel Virginia Bioinformatics Institute and Department of Biological Sciences, Virginia Tech, Washington Street, MC 0477, 24061-0477, Blacksburg, VA, USA aut Journal of Molecular Evolution Springer-Verlag 73/5-6(2011-12-01), 316-324 0022-2844 73:5-6<316 2011 73 239 https://doi.org/10.1007/s00239-011-9478-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00239-011-9478-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Madison-Villar Mercedita UT Arlington, Arlington, TX, USA aut NATIONALLICENCE 700 1- Michalak Pawel Virginia Bioinformatics Institute and Department of Biological Sciences, Virginia Tech, Washington Street, MC 0477, 24061-0477, Blacksburg, VA, USA aut NATIONALLICENCE 773 0- Journal of Molecular Evolution Springer-Verlag 73/5-6(2011-12-01), 316-324 0022-2844 73:5-6<316 2011 73 239 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer