caa a22 4500 445821140 CHVBK 20180317145244.0 cr unu---uuuuu 170323e20110101xx s 000 0 eng 10.1007/s00239-010-9406-3 doi (NATIONALLICENCE)springer-10.1007/s00239-010-9406-3 Characterization of Maltase Clusters in the Genus Drosophila [Elektronische Daten] [Marek Gabriško, Štefan Janeček] To reveal evolutionary history of maltase gene family in the genus Drosophila, we undertook a bioinformatics study of maltase genes from available genomes of 12 Drosophila species. Molecular evolution of a closely related glycoside hydrolase, the α-amylase, in Drosophila has been extensively studied for a long time. The α-amylases were even used as a model of evolution of multigene families. On the other hand, maltase, i.e., the α-glucosidase, got only scarce attention. In this study, we, therefore, investigated spatial organization of the maltase genes in Drosophila genomes, compared the amino acid sequences of the encoded enzymes and analyzed the intron/exon composition of orthologous genes. We found that the Drosophila maltases are more numerous than previously thought (ten instead of three genes) and are localized in two clusters on two chromosomes (2L and 2R). To elucidate the approximate time line of evolution of the clusters, we estimated the order and dated duplication of all the 10 genes. Both clusters are the result of ancient series of subsequent duplication events, which took place from 352 to 61million years ago, i.e., well before speciation to extant Drosophila species. Also observed was a remarkable intron/exon composition diversity of particular maltase genes of these clusters, probably a result of independent intron loss after duplication of intron-rich gene ancestor, which emerged well before speciation in a common ancestor of all extant Drosophila species. Springer Science+Business Media, LLC, 2010 Molecular evolution nationallicence Maltase nationallicence Alpha-amylase family nationallicence Gene cluster nationallicence Drosophila nationallicence Intron/exon composition nationallicence CAZy : Carbohydrate-Active enZymes nationallicence CSR : Conserved sequence region nationallicence EST : Expressed sequence tags nationallicence GH : Glycoside hydrolase nationallicence kb : Kilo base nationallicence LRT : Likelihood-ratio test nationallicence ML : Maximum likelihood nationallicence MP : Maximum parsimony nationallicence MYA : Million years ago nationallicence NJ : Neighbor-joining nationallicence S.E. : Standard error nationallicence Gabriško Marek Institute of Molecular Biology, Slovak Academy of Sciences, Dúbravská cesta 21, 84551, Bratislava, Slovakia aut Janeček Štefan Institute of Molecular Biology, Slovak Academy of Sciences, Dúbravská cesta 21, 84551, Bratislava, Slovakia aut Journal of Molecular Evolution Springer-Verlag 72/1(2011-01-01), 104-118 0022-2844 72:1<104 2011 72 239 https://doi.org/10.1007/s00239-010-9406-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00239-010-9406-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gabriško Marek Institute of Molecular Biology, Slovak Academy of Sciences, Dúbravská cesta 21, 84551, Bratislava, Slovakia aut NATIONALLICENCE 700 1- Janeček Štefan Institute of Molecular Biology, Slovak Academy of Sciences, Dúbravská cesta 21, 84551, Bratislava, Slovakia aut NATIONALLICENCE 773 0- Journal of Molecular Evolution Springer-Verlag 72/1(2011-01-01), 104-118 0022-2844 72:1<104 2011 72 239 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer