caa a22 4500 445821167 CHVBK 20180317145244.0 cr unu---uuuuu 170323e20110101xx s 000 0 eng 10.1007/s00239-010-9404-5 doi (NATIONALLICENCE)springer-10.1007/s00239-010-9404-5 Male Sex Interspecies Divergence and Down Regulation of Expression of Spermatogenesis Genes in Drosophila Sterile Hybrids [Elektronische Daten] [Vignesh Sundararajan, Alberto Civetta] Male sex genes have shown a pattern of rapid interspecies divergence at both the coding and gene expression level. A common outcome from crosses between closely-related species is hybrid male sterility. Phenotypic and genetic studies in Drosophila sterile hybrid males have shown that spermatogenesis arrest is postmeiotic with few exceptions, and that most misregulated genes are involved in late stages of spermatogenesis. Comparative studies of gene regulation in sterile hybrids and parental species have mainly used microarrays providing a whole genome representation of regulatory problems in sterile hybrids. Real-time PCR studies can reject or reveal differences not observed in microarray assays. Moreover, differences in gene expression between samples can be dependant on the source of RNA (e.g., whole body vs. tissue). Here we survey expression in D. simulans, D. mauritiana and both intra and interspecies hybrids using a real-time PCR approach for eight genes expressed at the four main stages of sperm development. We find that all genes show a trend toward under expression in the testes of sterile hybrids relative to parental species with only the two proliferation genes (bam and bgcn) and the two meiotic class genes (can and sa) showing significant down regulation. The observed pattern of down regulation for the genes tested can not fully explain hybrid male sterility. We discuss the down regulation of spermatogenesis genes in hybrids between closely-related species within the contest of rapid divergence experienced by the male genome, hybrid sterility and possible allometric changes due to subtle testes-specific developmental abnormalities. Springer Science+Business Media, LLC, 2010 Male sex nationallicence Gene expression nationallicence Sterility nationallicence Spermatogenesis nationallicence Sundararajan Vignesh Department of Biology, University of Winnipeg, 515 Portage Ave., R3B 2E9, Winnipeg, MB, Canada aut Civetta Alberto Department of Biology, University of Winnipeg, 515 Portage Ave., R3B 2E9, Winnipeg, MB, Canada aut Journal of Molecular Evolution Springer-Verlag 72/1(2011-01-01), 80-89 0022-2844 72:1<80 2011 72 239 https://doi.org/10.1007/s00239-010-9404-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00239-010-9404-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sundararajan Vignesh Department of Biology, University of Winnipeg, 515 Portage Ave., R3B 2E9, Winnipeg, MB, Canada aut NATIONALLICENCE 700 1- Civetta Alberto Department of Biology, University of Winnipeg, 515 Portage Ave., R3B 2E9, Winnipeg, MB, Canada aut NATIONALLICENCE 773 0- Journal of Molecular Evolution Springer-Verlag 72/1(2011-01-01), 80-89 0022-2844 72:1<80 2011 72 239 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer