caa a22 4500 445821175 CHVBK 20180317145244.0 cr unu---uuuuu 170323e20110101xx s 000 0 eng 10.1007/s00239-010-9400-9 doi (NATIONALLICENCE)springer-10.1007/s00239-010-9400-9 Proteome Evolution and the Metabolic Origins of Translation and Cellular Life [Elektronische Daten] [Derek Caetano-Anollés, Kyung Kim, Jay Mittenthal, Gustavo Caetano-Anollés] The origin of life has puzzled molecular scientists for over half a century. Yet fundamental questions remain unanswered, including which came first, the metabolic machinery or the encoding nucleic acids. In this study we take a protein-centric view and explore the ancestral origins of proteins. Protein domain structures in proteomes are highly conserved and embody molecular functions and interactions that are needed for cellular and organismal processes. Here we use domain structure to study the evolution of molecular function in the protein world. Timelines describing the age and function of protein domains at fold, fold superfamily, and fold family levels of structural complexity were derived from a structural phylogenomic census in hundreds of fully sequenced genomes. These timelines unfold congruent hourglass patterns in rates of appearance of domain structures and functions, functional diversity, and hierarchical complexity, and revealed a gradual build up of protein repertoires associated with metabolism, translation and DNA, in that order. The most ancient domain architectures were hydrolase enzymes and the first translation domains had catalytic functions for the aminoacylation and the molecular switch-driven transport of RNA. Remarkably, the most ancient domains had metabolic roles, did not interact with RNA, and preceded the gradual build-up of translation. In fact, the first translation domains had also a metabolic origin and were only later followed by specialized translation machinery. Our results explain how the generation of structure in the protein world and the concurrent crystallization of translation and diversified cellular life created further opportunities for proteomic diversification. Springer Science+Business Media, LLC, 2010 Origin of life nationallicence Phylogenetic analysis nationallicence Protein domain structure nationallicence Ribonucleoprotein world nationallicence RNA world nationallicence aRS : Aminoacyl-tRNA synthetase nationallicence F : Fold nationallicence FSF : Fold superfamily nationallicence FF : Fold family nationallicence Nd : Node distance nationallicence r-Protein : Ribosomal protein nationallicence SCOP : Structural classification of proteins nationallicence Caetano-Anollés Derek Evolutionary Bioinformatics Laboratory, Department of Crop Sciences, University of Illinois, 332 NSRC, 1101W Peabody Drive, 61801, Urbana, IL, USA aut Kim Kyung Evolutionary Bioinformatics Laboratory, Department of Crop Sciences, University of Illinois, 332 NSRC, 1101W Peabody Drive, 61801, Urbana, IL, USA aut Mittenthal Jay Department of Cell and Developmental Biology, University of Illinois, 61801, Urbana, IL, USA aut Caetano-Anollés Gustavo Evolutionary Bioinformatics Laboratory, Department of Crop Sciences, University of Illinois, 332 NSRC, 1101W Peabody Drive, 61801, Urbana, IL, USA aut Journal of Molecular Evolution Springer-Verlag 72/1(2011-01-01), 14-33 0022-2844 72:1<14 2011 72 239 https://doi.org/10.1007/s00239-010-9400-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00239-010-9400-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Caetano-Anollés Derek Evolutionary Bioinformatics Laboratory, Department of Crop Sciences, University of Illinois, 332 NSRC, 1101W Peabody Drive, 61801, Urbana, IL, USA aut NATIONALLICENCE 700 1- Kim Kyung Evolutionary Bioinformatics Laboratory, Department of Crop Sciences, University of Illinois, 332 NSRC, 1101W Peabody Drive, 61801, Urbana, IL, USA aut NATIONALLICENCE 700 1- Mittenthal Jay Department of Cell and Developmental Biology, University of Illinois, 61801, Urbana, IL, USA aut NATIONALLICENCE 700 1- Caetano-Anollés Gustavo Evolutionary Bioinformatics Laboratory, Department of Crop Sciences, University of Illinois, 332 NSRC, 1101W Peabody Drive, 61801, Urbana, IL, USA aut NATIONALLICENCE 773 0- Journal of Molecular Evolution Springer-Verlag 72/1(2011-01-01), 14-33 0022-2844 72:1<14 2011 72 239 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer