caa a22 4500 44582123X CHVBK 20180317145244.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s00239-011-9435-6 doi (NATIONALLICENCE)springer-10.1007/s00239-011-9435-6 Copia Retrotransposon in the Zaprionus Genus: Another Case of Transposable Element Sharing with the Drosophila melanogaster Subgroup [Elektronische Daten] [Nathalia de Setta, Marie-Anne Van Sluys, Pierre Capy, Claudia Carareto] Copia is a retrotransposon that appears to be distributed widely among the Drosophilidae subfamily. Evolutionary analyses of regulatory regions have indicated that the Copia retrotransposon evolved through both positive and purifying selection, and that horizontal transfer (HT) could also explain its patchy distribution of the among the subfamilies of the melanogaster subgroup. Additionally, Copia elements could also have transferred between melanogaster subgroup and other species of Drosophilidae—D. willistoni and Z. tuberculatus. In this study, we surveyed seven species of the Zaprionus genus by sequencing the LTR-ULR and reverse transcriptase regions, and by using RT-PCR in order to understand the distribution and evolutionary history of Copia in the Zaprionus genus. The Copia element was detected, and was transcriptionally active, in all species investigated. Structural and selection analysis revealed Zaprionus elements to be closely related to the most ancient subfamily of the melanogaster subgroup, and they seem to be evolving mainly under relaxed purifying selection. Taken together, these results allowed us to classify the Zaprionus sequences as a new subfamily—ZapCopia, a member of the Copia retrotransposon family of the melanogaster subgroup. These findings indicate that the Copia retrotransposon is an ancient component of the genomes of the Zaprionus species and broaden our understanding of the diversity of retrotransposons in the Zaprionus genus. Springer Science+Business Media, LLC, 2011 Copia nationallicence Retrotransposon nationallicence Zaprionus nationallicence Drosophila melanogaster nationallicence Evolution nationallicence Phylogeny nationallicence de Setta Nathalia Laboratory of Molecular Evolution, Department of Biology, UNESP, São Paulo State University, 15054-000, São José do Rio Preto, SP, Brazil aut Van Sluys Marie-Anne Genomics and Transposable Elements Lab, Department of Botany, São Paulo University (USP), 05508-090, São Paulo, SP, Brazil aut Capy Pierre Laboratoire Evolution, Génomes et Spéciation, UPR9034, CNRS, Gif-sur-Yvette, 91198, Paris, France aut Carareto Claudia Laboratory of Molecular Evolution, Department of Biology, UNESP, São Paulo State University, 15054-000, São José do Rio Preto, SP, Brazil aut Journal of Molecular Evolution Springer-Verlag 72/3(2011-03-01), 326-338 0022-2844 72:3<326 2011 72 239 https://doi.org/10.1007/s00239-011-9435-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00239-011-9435-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- de Setta Nathalia Laboratory of Molecular Evolution, Department of Biology, UNESP, São Paulo State University, 15054-000, São José do Rio Preto, SP, Brazil aut NATIONALLICENCE 700 1- Van Sluys Marie-Anne Genomics and Transposable Elements Lab, Department of Botany, São Paulo University (USP), 05508-090, São Paulo, SP, Brazil aut NATIONALLICENCE 700 1- Capy Pierre Laboratoire Evolution, Génomes et Spéciation, UPR9034, CNRS, Gif-sur-Yvette, 91198, Paris, France aut NATIONALLICENCE 700 1- Carareto Claudia Laboratory of Molecular Evolution, Department of Biology, UNESP, São Paulo State University, 15054-000, São José do Rio Preto, SP, Brazil aut NATIONALLICENCE 773 0- Journal of Molecular Evolution Springer-Verlag 72/3(2011-03-01), 326-338 0022-2844 72:3<326 2011 72 239 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer