caa a22 4500 445821302 CHVBK 20180317145245.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s00239-011-9451-6 doi (NATIONALLICENCE)springer-10.1007/s00239-011-9451-6 Molecular Evolution of Mollusc Shell Proteins: Insights from Proteomic Analysis of the Edible Mussel Mytilus [Elektronische Daten] [Benjamin Marie, Nathalie Le Roy, Isabelle Zanella-Cléon, Michel Becchi, Frédéric Marin] Shell matrix proteins (SMPs) that are embedded within calcified layers of mollusc shells are believed to play an essential role in controlling the biomineral synthesis and in increasing its mechanical properties. Among the wide diversity of mollusc shell textures, nacro-prismatic shells represent a tremendous opportunity for the investigation of the SMP evolution. Indeed, nacro-prismatic texture appears early in Cambrian molluscs and is still present in the shell of some bivalves, gastropods, cephalopods and very likely also, of some monoplacophorans. One key question is to know whether these shells are constructed from similar matrix protein assemblages, i.e. whether they share a common origin. Most of the molecular data published so far are restricted to two genera, the bivalve Pinctada and the gastropod Haliotis. The shell protein content of these two genera are clearly different, suggesting independent origins or considerable genetic drift from a common ancestor. In order to describe putatively conserved mollusc shell proteins, here we have investigated the SMP set of a new bivalve model belonging to another genera, the edible mussel Mytilus, using an up-to-date proteomic approach based on the interrogation of more than 70,000 EST sequences, recently available from NCBI public databases. We describe nine novel SMPs, among which three are completely novel, four are homologues of Pinctada SMPs and two are very likely homologues of Haliotis SMPs. This latter result constitutes the first report of conserved SMPs between bivalves and gastropods. More generally, our data suggest that mollusc SMP set may follow a mosaic pattern within the different mollusc models (Mytilus, Pinctada, Haliotis). We discuss the function of such proteins in calcifying matrices, the molecular evolution of SMP genes and the origin of mollusc nacro-prismatic SMPs. Springer Science+Business Media, LLC, 2011 Biomineralization nationallicence Proteomics nationallicence Mollusc shell nacre nationallicence Organic matrix nationallicence Evolution nationallicence Marie Benjamin UMR 5561 CNRS Biogéosciences, Université de Bourgogne, 6 bd. Gabriel, 21000, Dijon, France aut Le Roy Nathalie UMR 5561 CNRS Biogéosciences, Université de Bourgogne, 6 bd. Gabriel, 21000, Dijon, France aut Zanella-Cléon Isabelle IFR 128 BioSciences Gerland-Lyon Sud, UMR 5086 CNRS, Institut de Biologie et Chimie des Protéines, Université de Lyon 1, Lyon, France aut Becchi Michel IFR 128 BioSciences Gerland-Lyon Sud, UMR 5086 CNRS, Institut de Biologie et Chimie des Protéines, Université de Lyon 1, Lyon, France aut Marin Frédéric UMR 5561 CNRS Biogéosciences, Université de Bourgogne, 6 bd. Gabriel, 21000, Dijon, France aut Journal of Molecular Evolution Springer-Verlag 72/5-6(2011-06-01), 531-546 0022-2844 72:5-6<531 2011 72 239 https://doi.org/10.1007/s00239-011-9451-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00239-011-9451-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Marie Benjamin UMR 5561 CNRS Biogéosciences, Université de Bourgogne, 6 bd. Gabriel, 21000, Dijon, France aut NATIONALLICENCE 700 1- Le Roy Nathalie UMR 5561 CNRS Biogéosciences, Université de Bourgogne, 6 bd. Gabriel, 21000, Dijon, France aut NATIONALLICENCE 700 1- Zanella-Cléon Isabelle IFR 128 BioSciences Gerland-Lyon Sud, UMR 5086 CNRS, Institut de Biologie et Chimie des Protéines, Université de Lyon 1, Lyon, France aut NATIONALLICENCE 700 1- Becchi Michel IFR 128 BioSciences Gerland-Lyon Sud, UMR 5086 CNRS, Institut de Biologie et Chimie des Protéines, Université de Lyon 1, Lyon, France aut NATIONALLICENCE 700 1- Marin Frédéric UMR 5561 CNRS Biogéosciences, Université de Bourgogne, 6 bd. Gabriel, 21000, Dijon, France aut NATIONALLICENCE 773 0- Journal of Molecular Evolution Springer-Verlag 72/5-6(2011-06-01), 531-546 0022-2844 72:5-6<531 2011 72 239 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer