caa a22 4500 44582137X CHVBK 20180317145245.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s00239-011-9438-3 doi (NATIONALLICENCE)springer-10.1007/s00239-011-9438-3 Novel Hydrophobins from Trichoderma Define a New Hydrophobin Subclass: Protein Properties, Evolution, Regulation and Processing [Elektronische Daten] [Verena Seidl-Seiboth, Sabine Gruber, Ugur Sezerman, Torsten Schwecke, Aydin Albayrak, Torsten Neuhof, Hans von Döhren, Scott Baker, Christian Kubicek] Hydrophobins are small proteins, characterised by the presence of eight positionally conserved cysteine residues, and are present in all filamentous asco- and basidiomycetes. They are found on the outer surfaces of cell walls of hyphae and conidia, where they mediate interactions between the fungus and the environment. Hydrophobins are conventionally grouped into two classes (class I and II) according to their solubility in solvents, hydropathy profiles and spacing between the conserved cysteines. Here we describe a novel set of hydrophobins from Trichoderma spp. that deviate from this classification in their hydropathy, cysteine spacing and protein surface pattern. Phylogenetic analysis shows that they form separate clades within ascomycete class I hydrophobins. Using T. atroviride as a model, the novel hydrophobins were found to be expressed under conditions of glucose limitation and to be regulated by differential splicing. Springer Science+Business Media, LLC, 2011 Hydrophobin nationallicence Trichoderma nationallicence Protein evolution nationallicence Protein processing nationallicence Peptidomics nationallicence Splicing nationallicence Hypocrea nationallicence Seidl-Seiboth Verena Research Area Gene Technology and Applied Biochemistry, Institute of Chemical Engineering, Vienna University of Technology, Gumpendorferstrasse 1a/166-5, 1060, Vienna, Austria aut Gruber Sabine Research Area Gene Technology and Applied Biochemistry, Institute of Chemical Engineering, Vienna University of Technology, Gumpendorferstrasse 1a/166-5, 1060, Vienna, Austria aut Sezerman Ugur Biological Sciences and Bioengineering, Sabanci University, Orhanli, Tuzla, Istanbul, Turkey aut Schwecke Torsten Anagnostec GmbH, Im Biotechnologiepark TGZ II, 14943, Luckenwalde, Germany aut Albayrak Aydin Biological Sciences and Bioengineering, Sabanci University, Orhanli, Tuzla, Istanbul, Turkey aut Neuhof Torsten Anagnostec GmbH, Im Biotechnologiepark TGZ II, 14943, Luckenwalde, Germany aut von Döhren Hans TU Berlin, Institut für Chemie, FG Biochemie und Molekulare Biologie, Franklinstr. 29, 10587, Berlin, Germany aut Baker Scott Fungal Biotechnology Team, Chemical and Biological Process Development Group, Pacific Northwest National Laboratory, 902 Battelle Blvd., 99352, Richland, WA, USA aut Kubicek Christian Research Area Gene Technology and Applied Biochemistry, Institute of Chemical Engineering, Vienna University of Technology, Gumpendorferstrasse 1a/166-5, 1060, Vienna, Austria aut Journal of Molecular Evolution Springer-Verlag 72/4(2011-04-01), 339-351 0022-2844 72:4<339 2011 72 239 https://doi.org/10.1007/s00239-011-9438-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00239-011-9438-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Seidl-Seiboth Verena Research Area Gene Technology and Applied Biochemistry, Institute of Chemical Engineering, Vienna University of Technology, Gumpendorferstrasse 1a/166-5, 1060, Vienna, Austria aut NATIONALLICENCE 700 1- Gruber Sabine Research Area Gene Technology and Applied Biochemistry, Institute of Chemical Engineering, Vienna University of Technology, Gumpendorferstrasse 1a/166-5, 1060, Vienna, Austria aut NATIONALLICENCE 700 1- Sezerman Ugur Biological Sciences and Bioengineering, Sabanci University, Orhanli, Tuzla, Istanbul, Turkey aut NATIONALLICENCE 700 1- Schwecke Torsten Anagnostec GmbH, Im Biotechnologiepark TGZ II, 14943, Luckenwalde, Germany aut NATIONALLICENCE 700 1- Albayrak Aydin Biological Sciences and Bioengineering, Sabanci University, Orhanli, Tuzla, Istanbul, Turkey aut NATIONALLICENCE 700 1- Neuhof Torsten Anagnostec GmbH, Im Biotechnologiepark TGZ II, 14943, Luckenwalde, Germany aut NATIONALLICENCE 700 1- von Döhren Hans TU Berlin, Institut für Chemie, FG Biochemie und Molekulare Biologie, Franklinstr. 29, 10587, Berlin, Germany aut NATIONALLICENCE 700 1- Baker Scott Fungal Biotechnology Team, Chemical and Biological Process Development Group, Pacific Northwest National Laboratory, 902 Battelle Blvd., 99352, Richland, WA, USA aut NATIONALLICENCE 700 1- Kubicek Christian Research Area Gene Technology and Applied Biochemistry, Institute of Chemical Engineering, Vienna University of Technology, Gumpendorferstrasse 1a/166-5, 1060, Vienna, Austria aut NATIONALLICENCE 773 0- Journal of Molecular Evolution Springer-Verlag 72/4(2011-04-01), 339-351 0022-2844 72:4<339 2011 72 239 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer