caa a22 4500 445821388 CHVBK 20180317145245.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s00239-011-9439-2 doi (NATIONALLICENCE)springer-10.1007/s00239-011-9439-2 Insertion Sequence-Driven Evolution of Escherichia coli in Chemostats [Elektronische Daten] [Joël Gaffé, Christopher McKenzie, Ram Maharjan, Evelyne Coursange, Tom Ferenci, Dominique Schneider] Insertion sequence (IS) elements are present in almost all bacterial genomes and are mobile enough to provide genomic tools to differentiate closely related isolates. They can be used to estimate genetic diversity and identify fitness-enhancing mutations during evolution experiments. Here, we determined the genomic distribution of eight IS elements in 120 genomes sampled from Escherichia coli populations that evolved in glucose- and phosphate-limited chemostats by comparison to the ancestral pattern. No significant differential transposition of the various IS types was detected across the environments. The phylogenies revealed substantial diversity amongst clones sampled from each chemostat, consistent with the phenotypic diversity within populations. Two IS-related changes were common to independent chemostats, suggesting parallel evolution. One of them corresponded to insertions of IS1 elements within rpoS encoding the master regulator of stress conditions. The other parallel event was an IS5-dependent deletion including mutY involved in DNA repair, thereby providing the molecular mechanism of generation of mutator clones in these evolving populations. These deletions occurred in different co-existing genotypes within single populations and were of various sizes. Moreover, differential locations of IS elements combined with their transpositional activity provided evolved clones with different phenotypic landscapes. Therefore, IS elements strongly influenced the evolutionary processes in continuous E. coli cultures by providing a way to modify both the global regulatory network and the mutation rates of evolving cells. Springer Science+Business Media, LLC, 2011 Evolution nationallicence Insertion sequence nationallicence Transposition nationallicence Rearrangements nationallicence Escherichia coli nationallicence Genetic diversity nationallicence Chemostats nationallicence Gaffé Joël Laboratoire Adaptation et Pathogénie des Microorganismes, Université Joseph Fourier Grenoble 1, BP 170, 38042, Grenoble Cedex 9, France aut McKenzie Christopher School of Molecular Bioscience, The University of Sydney, Sydney, NSW, Australia aut Maharjan Ram School of Molecular Bioscience, The University of Sydney, Sydney, NSW, Australia aut Coursange Evelyne Laboratoire Adaptation et Pathogénie des Microorganismes, Université Joseph Fourier Grenoble 1, BP 170, 38042, Grenoble Cedex 9, France aut Ferenci Tom School of Molecular Bioscience, The University of Sydney, Sydney, NSW, Australia aut Schneider Dominique Laboratoire Adaptation et Pathogénie des Microorganismes, Université Joseph Fourier Grenoble 1, BP 170, 38042, Grenoble Cedex 9, France aut Journal of Molecular Evolution Springer-Verlag 72/4(2011-04-01), 398-412 0022-2844 72:4<398 2011 72 239 https://doi.org/10.1007/s00239-011-9439-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00239-011-9439-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gaffé Joël Laboratoire Adaptation et Pathogénie des Microorganismes, Université Joseph Fourier Grenoble 1, BP 170, 38042, Grenoble Cedex 9, France aut NATIONALLICENCE 700 1- McKenzie Christopher School of Molecular Bioscience, The University of Sydney, Sydney, NSW, Australia aut NATIONALLICENCE 700 1- Maharjan Ram School of Molecular Bioscience, The University of Sydney, Sydney, NSW, Australia aut NATIONALLICENCE 700 1- Coursange Evelyne Laboratoire Adaptation et Pathogénie des Microorganismes, Université Joseph Fourier Grenoble 1, BP 170, 38042, Grenoble Cedex 9, France aut NATIONALLICENCE 700 1- Ferenci Tom School of Molecular Bioscience, The University of Sydney, Sydney, NSW, Australia aut NATIONALLICENCE 700 1- Schneider Dominique Laboratoire Adaptation et Pathogénie des Microorganismes, Université Joseph Fourier Grenoble 1, BP 170, 38042, Grenoble Cedex 9, France aut NATIONALLICENCE 773 0- Journal of Molecular Evolution Springer-Verlag 72/4(2011-04-01), 398-412 0022-2844 72:4<398 2011 72 239 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer