caa a22 4500 445821426 CHVBK 20180317145245.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s00239-011-9436-5 doi (NATIONALLICENCE)springer-10.1007/s00239-011-9436-5 RrS1 -like Sequences of Water Frogs from Central Europe and Around the Aegean Sea: Chromosomal Organization, Evolution, Possible Function [Elektronische Daten] [Silvia Marracci, Valentina Michelotti, Gaston-Denis Guex, Hansjürg Hotz, Thomas Uzzell, Matilde Ragghianti] RrS1-like sequences of water frogs (genus Pelophylax) display varied genomic organization, whereas the centromeric hybridization pattern reveals species-specific differences. Using fluorescent in situ hybridization, Pelophylax cf. bedriagae, Pelophylaxkurtmuelleri, and Pelophylaxridibundus showed a hybridization signal at centromeres of chromosomes 1-5, but in P. kurtmuelleri the medium-small chromosome labeled was 10 rather than 8. Pelophylaxcretensis had almost 16 of 26 centromeres labeled, as did Pelophylaxlessonae from Poland when its chromosomes are hybridized with a homologous probe. When StuI-digested genomic DNA was hybridized with RrS1 probe, hybridization ladders for P. ridibundus from Poland have evenly spaced steps (about 100bp) of uniform intensity from about 200bp upward. Steps in hybridization ladders from circum-Aegean taxa vary in intensity: larger, odd-numbered steps are often fainter. A strong double band (800/900bp) in Anatolian P. cf. bedriagae, emphasized by a weak 700bp band, distinguishes them from P. kurtmuelleri from the Peloponnisos, in which the 900bp band is almost absent. The ladder in P. cretensis lacks odd-numbered steps. A and B repeats, observed originally within the RrS1 satellite of P. ridibundus, occur also in the circum-Aegean frogs and in P. lessonae, Pelophylaxepeiroticus, Pelophylaxsaharicus, and Pelophylaxshqipericus. It is plausible that AB dimers or ABB trimers rather than A or B monomers correspond to functional/evolutionary units. The presence of regions similar to yeast CDEs and mammalian CENP-B boxes suggests a role for RrS1 sequences in centromere organization. Springer Science+Business Media, LLC, 2011 Centromeric satellite nationallicence RrS1 DNA element nationallicence CDE-like element nationallicence CENP-B box-like element nationallicence Pelophylax nationallicence Western Palearctic water frog nationallicence Marracci Silvia Laboratori di Biologia cellulare e dello sviluppo, Dipartimento di Biologia, Università di Pisa, Pisa, Italy aut Michelotti Valentina Laboratori di Biologia cellulare e dello sviluppo, Dipartimento di Biologia, Università di Pisa, Pisa, Italy aut Guex Gaston-Denis Institut für Evolutionsbiologie und Umweltwissenschaften, Universität Zürich, Zürich, Switzerland aut Hotz Hansjürg Institut für Evolutionsbiologie und Umweltwissenschaften, Universität Zürich, Zürich, Switzerland aut Uzzell Thomas Center for Systematic Biology and Evolution, Academy of Natural Sciences, 1900 Benjamin Franklin Parkway, 19103, Philadelphia, PA, USA aut Ragghianti Matilde Laboratori di Biologia cellulare e dello sviluppo, Dipartimento di Biologia, Università di Pisa, Pisa, Italy aut Journal of Molecular Evolution Springer-Verlag 72/4(2011-04-01), 368-382 0022-2844 72:4<368 2011 72 239 https://doi.org/10.1007/s00239-011-9436-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00239-011-9436-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Marracci Silvia Laboratori di Biologia cellulare e dello sviluppo, Dipartimento di Biologia, Università di Pisa, Pisa, Italy aut NATIONALLICENCE 700 1- Michelotti Valentina Laboratori di Biologia cellulare e dello sviluppo, Dipartimento di Biologia, Università di Pisa, Pisa, Italy aut NATIONALLICENCE 700 1- Guex Gaston-Denis Institut für Evolutionsbiologie und Umweltwissenschaften, Universität Zürich, Zürich, Switzerland aut NATIONALLICENCE 700 1- Hotz Hansjürg Institut für Evolutionsbiologie und Umweltwissenschaften, Universität Zürich, Zürich, Switzerland aut NATIONALLICENCE 700 1- Uzzell Thomas Center for Systematic Biology and Evolution, Academy of Natural Sciences, 1900 Benjamin Franklin Parkway, 19103, Philadelphia, PA, USA aut NATIONALLICENCE 700 1- Ragghianti Matilde Laboratori di Biologia cellulare e dello sviluppo, Dipartimento di Biologia, Università di Pisa, Pisa, Italy aut NATIONALLICENCE 773 0- Journal of Molecular Evolution Springer-Verlag 72/4(2011-04-01), 368-382 0022-2844 72:4<368 2011 72 239 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer