caa a22 4500 445821450 CHVBK 20190327083712.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s00239-011-9431-x doi (NATIONALLICENCE)springer-10.1007/s00239-011-9431-x Identification and Characterization of Gene Expression Involved in the Coloration of Cichlid Fish Using Microarray and qRT-PCR Approaches [Elektronische Daten] [Helen Gunter, Céline Clabaut, Walter Salzburger, Axel Meyer] It has been suggested that speciation on the basis of sexual selection is an important mechanism for the generation of new species for East African cichlids, where male body coloration is one of the major discriminatory factors used by females in mate choice. To gain insight into the molecular basis of cichlid coloration, we studied the Lake Malawi cichlid Pseudotropheus saulosi, comparing transcription in the bright blue skin of males to the yellow skin of females. Our cDNA microarray experiments identified 46 clones that exhibited expression differences between the two sexes, of which five were confirmed to be differentially expressed by relative quantitative real-time PCR (qRT-PCR). This gene list includes a representative from the endosomal-to-Golgi vesicle trafficking pathway, Coatomer protein complex, subunit zeta-1 (Copz-1), which is known to be a critical determinant of pigmentation in humans and zebrafish. With the support of microscopic images of the skin of these specimens, we interpret the transcriptional differences between the blue males and yellow females. Here, we provide insight into the putative functional diversification of genes involved in the coloration of cichlids and by extension, on the evolution of coloration in teleost fish. Springer Science+Business Media, LLC, 2011 Adaptive evolution nationallicence Cichlid species flocks nationallicence Sexual selection nationallicence Copz nationallicence Collagen 1 alpha nationallicence Gunter Helen Lehrstuhl für Zoologie und Evolutionsbiologie, Department of Biology, University of Konstanz, Universitätsstr. 10, 78457, Constance, Germany aut Clabaut Céline Lehrstuhl für Zoologie und Evolutionsbiologie, Department of Biology, University of Konstanz, Universitätsstr. 10, 78457, Constance, Germany aut Salzburger Walter Lehrstuhl für Zoologie und Evolutionsbiologie, Department of Biology, University of Konstanz, Universitätsstr. 10, 78457, Constance, Germany aut Meyer Axel Lehrstuhl für Zoologie und Evolutionsbiologie, Department of Biology, University of Konstanz, Universitätsstr. 10, 78457, Constance, Germany aut Journal of Molecular Evolution Springer-Verlag 72/2(2011-02-01), 127-137 0022-2844 72:2<127 2011 72 239 https://doi.org/10.1007/s00239-011-9431-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00239-011-9431-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gunter Helen Lehrstuhl für Zoologie und Evolutionsbiologie, Department of Biology, University of Konstanz, Universitätsstr. 10, 78457, Constance, Germany aut NATIONALLICENCE 700 1- Clabaut Céline Lehrstuhl für Zoologie und Evolutionsbiologie, Department of Biology, University of Konstanz, Universitätsstr. 10, 78457, Constance, Germany aut NATIONALLICENCE 700 1- Salzburger Walter Lehrstuhl für Zoologie und Evolutionsbiologie, Department of Biology, University of Konstanz, Universitätsstr. 10, 78457, Constance, Germany aut NATIONALLICENCE 700 1- Meyer Axel Lehrstuhl für Zoologie und Evolutionsbiologie, Department of Biology, University of Konstanz, Universitätsstr. 10, 78457, Constance, Germany aut NATIONALLICENCE 773 0- Journal of Molecular Evolution Springer-Verlag 72/2(2011-02-01), 127-137 0022-2844 72:2<127 2011 72 239 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer