caa a22 4500 445821493 CHVBK 20180317145245.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s00239-010-9424-1 doi (NATIONALLICENCE)springer-10.1007/s00239-010-9424-1 Isolation and Characterization of the CYP2D6 Gene in Felidae with Comparison to Other Mammals [Elektronische Daten] [Tamara Schenekar, Kathrin Winkler, Jennifer Troyer, Steven Weiss] The highly polymorphic CYP2D6 protein metabolizes about 25% of commonly used drugs and underlies a broad spectrum of drug responses among individuals. In contrast to extensive knowledge on the human CYP2D6 gene, little is known about the gene in non-human mammals. CYP2D6 mRNA from 23 cats (Felidae) spanning seven species were compared to available CYPD6 sequences in ten additional mammals and multiple allelic variants in humans. A relatively high mean dN/dS ratio (0.565) was observed, especially within Felidae. Pairwise dN/dS ratios were non-monotonically distributed with respect to evolutionary distance suggesting either positive selection or retention of slightly deleterious mutations. Positive selection on specific codons, most notably in regions involved in substrate recognition and membrane anchoring is supported and the possible influence of diet on specific amino acid changes in substrate binding sites is discussed. Springer Science+Business Media, LLC, 2010 Cytochrome P450 nationallicence Cats nationallicence Selection nationallicence d N /d S nationallicence Slightly deleterious mutations nationallicence Schenekar Tamara Karl-Franzens Universität Graz, Institut für Zoologie, Universitätsplatz 2, A-8010, Graz, Austria aut Winkler Kathrin Karl-Franzens Universität Graz, Institut für Zoologie, Universitätsplatz 2, A-8010, Graz, Austria aut Troyer Jennifer Laboratory of Genomic Diversity, SAIC-Frederic, Inc., National Cancer Institute-Frederick, 21702, Frederick, MD, USA aut Weiss Steven Karl-Franzens Universität Graz, Institut für Zoologie, Universitätsplatz 2, A-8010, Graz, Austria aut Journal of Molecular Evolution Springer-Verlag 72/2(2011-02-01), 222-231 0022-2844 72:2<222 2011 72 239 https://doi.org/10.1007/s00239-010-9424-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00239-010-9424-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Schenekar Tamara Karl-Franzens Universität Graz, Institut für Zoologie, Universitätsplatz 2, A-8010, Graz, Austria aut NATIONALLICENCE 700 1- Winkler Kathrin Karl-Franzens Universität Graz, Institut für Zoologie, Universitätsplatz 2, A-8010, Graz, Austria aut NATIONALLICENCE 700 1- Troyer Jennifer Laboratory of Genomic Diversity, SAIC-Frederic, Inc., National Cancer Institute-Frederick, 21702, Frederick, MD, USA aut NATIONALLICENCE 700 1- Weiss Steven Karl-Franzens Universität Graz, Institut für Zoologie, Universitätsplatz 2, A-8010, Graz, Austria aut NATIONALLICENCE 773 0- Journal of Molecular Evolution Springer-Verlag 72/2(2011-02-01), 222-231 0022-2844 72:2<222 2011 72 239 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer