caa a22 4500 445821507 CHVBK 20180317145245.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s00239-010-9413-4 doi (NATIONALLICENCE)springer-10.1007/s00239-010-9413-4 Molecular Variability and Evolution of the Pectate Lyase ( pel -2) Parasitism Gene in Cyst Nematodes Parasitizing Different Solanaceous Plants [Elektronische Daten] [Barbara Geric Stare, Didier Fouville, Saša Širca, Aurore Gallot, Gregor Urek, Eric Grenier] While pectate lyases are major parasitism factors in plant-parasitic nematodes, there is little information on the variability of these genes within species and their utility as pathotype or host range molecular markers. We have analysed polymorphisms of pectate lyase 2 (pel-2) gene, which degrades the unesterified polygalacturonate (pectate) of the host cell-wall, in the genus Globodera. Molecular variability of the pel-2 gene and the predicted protein was evaluated in populations of G. rostochiensis, G. pallida, G. "mexicana” and G. tabacum. Seventy eight pel-2 sequences were obtained and aligned. Point mutations were observed at 373 positions, 57% of these affect the coding part of the gene and produce 129aa replacements. The observed polymorphism does not correlate either to the pathotypes proposed in potato cyst nematodes (PCN) or the subspecies described in tobacco cyst nematodes. The trees reveal a topology different from the admitted species topology as G. rostochiensis and G. pallida sequences are more similar to each other than to G. tabacum. Species-specific sites, potentially applicable for identification, and sites distinguishing PCN from tobacco cyst nematodes, were identified. As both G. rostochiensis and G. pallida display the same host range, but distinct from G. tabacum, which cannot parasitize potato plants, it is tempting to speculate that pel-2 genes polymorphism may be implicated in this adaptation, a view supported by the fact that no active pectate lyase 2 was found in G. "mexicana”, a close relative of G. pallida that is unable to develop on cultivated potato varieties. Springer Science+Business Media, LLC, 2010 Cell-wall degradation nationallicence Globodera nationallicence Parasitic factor nationallicence Pectate lyase nationallicence Plant-parasitic nematode nationallicence Potato cyst nematode nationallicence Convergent evolution nationallicence Purifying selection nationallicence Geric Stare Barbara Plant Protection Department, Agricultural Institute of Slovenia, Hacquetova 17, 1001, Ljubljana, Slovenia aut Fouville Didier INRA, UMR1099 BiO3P (Biology of Organisms and Populations Applied to Plant Protection), Domaine de la Motte, BP 35327, 35653, Le Rheu, France aut Širca Saša Plant Protection Department, Agricultural Institute of Slovenia, Hacquetova 17, 1001, Ljubljana, Slovenia aut Gallot Aurore INRA, UMR1099 BiO3P (Biology of Organisms and Populations Applied to Plant Protection), Domaine de la Motte, BP 35327, 35653, Le Rheu, France aut Urek Gregor Plant Protection Department, Agricultural Institute of Slovenia, Hacquetova 17, 1001, Ljubljana, Slovenia aut Grenier Eric INRA, UMR1099 BiO3P (Biology of Organisms and Populations Applied to Plant Protection), Domaine de la Motte, BP 35327, 35653, Le Rheu, France aut Journal of Molecular Evolution Springer-Verlag 72/2(2011-02-01), 169-181 0022-2844 72:2<169 2011 72 239 https://doi.org/10.1007/s00239-010-9413-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00239-010-9413-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Geric Stare Barbara Plant Protection Department, Agricultural Institute of Slovenia, Hacquetova 17, 1001, Ljubljana, Slovenia aut NATIONALLICENCE 700 1- Fouville Didier INRA, UMR1099 BiO3P (Biology of Organisms and Populations Applied to Plant Protection), Domaine de la Motte, BP 35327, 35653, Le Rheu, France aut NATIONALLICENCE 700 1- Širca Saša Plant Protection Department, Agricultural Institute of Slovenia, Hacquetova 17, 1001, Ljubljana, Slovenia aut NATIONALLICENCE 700 1- Gallot Aurore INRA, UMR1099 BiO3P (Biology of Organisms and Populations Applied to Plant Protection), Domaine de la Motte, BP 35327, 35653, Le Rheu, France aut NATIONALLICENCE 700 1- Urek Gregor Plant Protection Department, Agricultural Institute of Slovenia, Hacquetova 17, 1001, Ljubljana, Slovenia aut NATIONALLICENCE 700 1- Grenier Eric INRA, UMR1099 BiO3P (Biology of Organisms and Populations Applied to Plant Protection), Domaine de la Motte, BP 35327, 35653, Le Rheu, France aut NATIONALLICENCE 773 0- Journal of Molecular Evolution Springer-Verlag 72/2(2011-02-01), 169-181 0022-2844 72:2<169 2011 72 239 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer