caa a22 4500 445822864 CHVBK 20180317145249.0 cr unu---uuuuu 170323e20110101xx s 000 0 eng 10.1007/s10616-010-9318-5 doi (NATIONALLICENCE)springer-10.1007/s10616-010-9318-5 Effect of cocaine and crack on the ploidy status of Tetrahymena pyriformis [Elektronische Daten] a study using DNA image analysis [Maria Stefanidou, Vasiliki Hatzi, Georgia Terzoudi, Ariadni Loutsidou, Constantinos Maravelias] The effect of cocaine and crack on the ploidy status of Feulgen-stained Tetrahymena pyriformis macronuclei using computerized DNA image analysis system was tested. For this purpose, selected doses of 5, 10 and 20μg (per mL culture) of both drugs were administered for 2, 5 and 20h to protozoa cultures and DNA image analysis of T. pyriformis nuclei was performed. The analysis was based on the measurement of the following parameters: Ploidy Balance (PB), Degree of Aneuploidy (DA), skewness and kurtosis. The results have shown a positive effect of both cocaine and crack on PB and on DA of T. pyriformis macronuclei. In particular, our results reveal that the aneugenic effect (which is expressed as a decrease in PB and an increase in DA) of cocaine on T. pyriformis macronuclei follows a dose-dependent manner, while crack induces aneuploidy in a dose-independent manner. Changes in the PB and DA values would induce a disturbance in the cellular density and heterogeneity of chromatin and the increase in skewness and kurtosis values after exposure of T. pyriformis to both drugs, did confirm this hypothesis. These observations were further correlated with alterations in the chromosomal segregation and with damage in mitotic spindle microtubules observed previously. In this study the impact of cocaine and crack on genomic instability and carcinogenesis was further supported and T. pyriformis can be proposed as a model organism to test the nuclear ploidy status after exposure to harmful chemicals and drugs. Springer Science+Business Media B.V., 2010 Tetrahymena pyriformis nationallicence Cocaine nationallicence Crack nationallicence DNA image analysis nationallicence Ploidy balance nationallicence Aneuploidy nationallicence Stefanidou Maria Department of Forensic Medicine and Toxicology, Medical School, University of Athens, 75 M. Asias str., Goudi 115.27, Athens, Greece aut Hatzi Vasiliki Institute of Nuclear Technology & Radiation Protection, National Centre for Scientific Research, NCSR "Demokritos”, Ag. Paraskevi Attikis, Athens, Greece aut Terzoudi Georgia Institute of Radioisotopes & Radiodiagnostic Products, National Centre for Scientific Research, NCSR "Demokritos”, Ag. Paraskevi Attikis, Athens, Greece aut Loutsidou Ariadni Department of Forensic Medicine and Toxicology, Medical School, University of Athens, 75 M. Asias str., Goudi 115.27, Athens, Greece aut Maravelias Constantinos Department of Forensic Medicine and Toxicology, Medical School, University of Athens, 75 M. Asias str., Goudi 115.27, Athens, Greece aut Cytotechnology Springer Netherlands 63/1(2011-01-01), 35-40 0920-9069 63:1<35 2011 63 10616 https://doi.org/10.1007/s10616-010-9318-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10616-010-9318-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Stefanidou Maria Department of Forensic Medicine and Toxicology, Medical School, University of Athens, 75 M. Asias str., Goudi 115.27, Athens, Greece aut NATIONALLICENCE 700 1- Hatzi Vasiliki Institute of Nuclear Technology & Radiation Protection, National Centre for Scientific Research, NCSR "Demokritos”, Ag. Paraskevi Attikis, Athens, Greece aut NATIONALLICENCE 700 1- Terzoudi Georgia Institute of Radioisotopes & Radiodiagnostic Products, National Centre for Scientific Research, NCSR "Demokritos”, Ag. Paraskevi Attikis, Athens, Greece aut NATIONALLICENCE 700 1- Loutsidou Ariadni Department of Forensic Medicine and Toxicology, Medical School, University of Athens, 75 M. Asias str., Goudi 115.27, Athens, Greece aut NATIONALLICENCE 700 1- Maravelias Constantinos Department of Forensic Medicine and Toxicology, Medical School, University of Athens, 75 M. Asias str., Goudi 115.27, Athens, Greece aut NATIONALLICENCE 773 0- Cytotechnology Springer Netherlands 63/1(2011-01-01), 35-40 0920-9069 63:1<35 2011 63 10616 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer