caa a22 4500 445822872 CHVBK 20180317145249.0 cr unu---uuuuu 170323e20110101xx s 000 0 eng 10.1007/s10616-010-9315-8 doi (NATIONALLICENCE)springer-10.1007/s10616-010-9315-8 A multi-gene transcriptional profiling approach to the discovery of cell signature markers [Elektronische Daten] [Youichiro Wada, Dan Li, Anne Merley, Andrew Zukauskas, William Aird, Harold Dvorak, Shou-Ching Shih] A profile of transcript abundances from multiple genes constitutes a molecular signature if the expression pattern is unique to one cell type. Here we measure mRNA copy numbers per cell by normalizing per million copies of 18S rRNA and identify 6 genes (TIE1, KDR, CDH5, TIE2, EFNA1 and MYO5C) out of 79 genes tested as excellent molecular signature markers for endothelial cells (ECs) in vitro. The selected genes are uniformly expressed in ECs of 4 different origins but weakly or not expressed in 4 non-EC cell lines. A multi-gene transcriptional profile of these 6 genes clearly distinguishes ECs from non-ECs in vitro. We conclude that (i) a profile of mRNA copy numbers per cell from a well-chosen multi-gene panel can act as a sensitive and accurate cell type signature marker, and (ii) the method described here can be applied to in vivo cell fingerprinting and molecular diagnosis. Springer Science+Business Media B.V., 2010 Molecular signature nationallicence Gene expression profiling nationallicence mRNA copy numbers nationallicence Endothelial cells nationallicence Wada Youichiro Center for Vascular Biological Research and Division of Molecular and Vascular Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, 99 Brookline Avenue, 02215, Boston, MA, USA aut Li Dan Center for Vascular Biological Research and Department of Pathology, Beth Israel Deaconess Medical Center, Harvard Medical School, 99 Brookline Avenue, 02215, Boston, MA, USA aut Merley Anne Center for Vascular Biological Research and Department of Pathology, Beth Israel Deaconess Medical Center, Harvard Medical School, 99 Brookline Avenue, 02215, Boston, MA, USA aut Zukauskas Andrew Center for Vascular Biological Research and Department of Pathology, Beth Israel Deaconess Medical Center, Harvard Medical School, 99 Brookline Avenue, 02215, Boston, MA, USA aut Aird William Center for Vascular Biological Research and Division of Molecular and Vascular Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, 99 Brookline Avenue, 02215, Boston, MA, USA aut Dvorak Harold Center for Vascular Biological Research and Department of Pathology, Beth Israel Deaconess Medical Center, Harvard Medical School, 99 Brookline Avenue, 02215, Boston, MA, USA aut Shih Shou-Ching Center for Vascular Biological Research and Department of Pathology, Beth Israel Deaconess Medical Center, Harvard Medical School, 99 Brookline Avenue, 02215, Boston, MA, USA aut Cytotechnology Springer Netherlands 63/1(2011-01-01), 25-33 0920-9069 63:1<25 2011 63 10616 https://doi.org/10.1007/s10616-010-9315-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10616-010-9315-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wada Youichiro Center for Vascular Biological Research and Division of Molecular and Vascular Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, 99 Brookline Avenue, 02215, Boston, MA, USA aut NATIONALLICENCE 700 1- Li Dan Center for Vascular Biological Research and Department of Pathology, Beth Israel Deaconess Medical Center, Harvard Medical School, 99 Brookline Avenue, 02215, Boston, MA, USA aut NATIONALLICENCE 700 1- Merley Anne Center for Vascular Biological Research and Department of Pathology, Beth Israel Deaconess Medical Center, Harvard Medical School, 99 Brookline Avenue, 02215, Boston, MA, USA aut NATIONALLICENCE 700 1- Zukauskas Andrew Center for Vascular Biological Research and Department of Pathology, Beth Israel Deaconess Medical Center, Harvard Medical School, 99 Brookline Avenue, 02215, Boston, MA, USA aut NATIONALLICENCE 700 1- Aird William Center for Vascular Biological Research and Division of Molecular and Vascular Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, 99 Brookline Avenue, 02215, Boston, MA, USA aut NATIONALLICENCE 700 1- Dvorak Harold Center for Vascular Biological Research and Department of Pathology, Beth Israel Deaconess Medical Center, Harvard Medical School, 99 Brookline Avenue, 02215, Boston, MA, USA aut NATIONALLICENCE 700 1- Shih Shou-Ching Center for Vascular Biological Research and Department of Pathology, Beth Israel Deaconess Medical Center, Harvard Medical School, 99 Brookline Avenue, 02215, Boston, MA, USA aut NATIONALLICENCE 773 0- Cytotechnology Springer Netherlands 63/1(2011-01-01), 25-33 0920-9069 63:1<25 2011 63 10616 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer