caa a22 4500 445822880 CHVBK 20180317145249.0 cr unu---uuuuu 170323e20110101xx s 000 0 eng 10.1007/s10616-010-9314-9 doi (NATIONALLICENCE)springer-10.1007/s10616-010-9314-9 Evaluating osteochondral defect repair potential of autologous rabbit bone marrow cells on type II collagen scaffold [Elektronische Daten] [Wei-Chuan Chen, Chao-Ling Yao, Yu-Hong Wei, I-Ming Chu] The feasibility of using genipin cross-linked type II collagen scaffold with rabbit bone marrow mesenchymal stem cells (RBMSCs) to repair cartilage defect was herein studied. Induction of RBMSCs into chondrocytic phenotype on type II collagen scaffold in vitro was conducted using TGF-β 3 containing medium. After 3-weeks of induction, chondrocytic behavior, including marker genes expression and specific extracellular matrix (ECM) secretion, was observed. In the in vivo evaluation experiment, the scaffolds containing RBMSCs without prior induction were autologous implanted into the articular cartilage defects made by subchondral drilling. The repairing ability was evaluated. After 2months, chondrocyte-like cells with lacuna structure and corresponding ECM were found in the repaired sites without apparent inflammation. After 24weeks, we could easily find cartilage structure the same with normal cartilage in the repair site. In conclusion, it was shown that the scaffolds in combination of in vivo conditions can induce RBMSCs into chondrocytes in repaired area and would be a possible method for articular cartilage repair in clinic and cartilage tissue engineering. Springer Science+Business Media B.V., 2010 Cartilage defect nationallicence Type II collagen scaffold nationallicence Rabbit bone marrow mesenchymal stem cells nationallicence Chondrocyte-like cells nationallicence In vivo nationallicence Chen Wei-Chuan Department of Chemical Engineering, National Tsing Hua University, 101, Kuang-Fu Road, 300, Hsin-Chu City, Taiwan aut Yao Chao-Ling Department of Chemical Engineering and Materials Science, Yuan Ze University, 320, Chung-Li, Taoyuan, Taiwan aut Wei Yu-Hong Graduate School of Biotechnology and Bioengineering, Yuan Ze University, 320, Chung-Li, Taoyuan, Taiwan aut Chu I-Ming Department of Chemical Engineering, National Tsing Hua University, 101, Kuang-Fu Road, 300, Hsin-Chu City, Taiwan aut Cytotechnology Springer Netherlands 63/1(2011-01-01), 13-23 0920-9069 63:1<13 2011 63 10616 https://doi.org/10.1007/s10616-010-9314-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10616-010-9314-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chen Wei-Chuan Department of Chemical Engineering, National Tsing Hua University, 101, Kuang-Fu Road, 300, Hsin-Chu City, Taiwan aut NATIONALLICENCE 700 1- Yao Chao-Ling Department of Chemical Engineering and Materials Science, Yuan Ze University, 320, Chung-Li, Taoyuan, Taiwan aut NATIONALLICENCE 700 1- Wei Yu-Hong Graduate School of Biotechnology and Bioengineering, Yuan Ze University, 320, Chung-Li, Taoyuan, Taiwan aut NATIONALLICENCE 700 1- Chu I-Ming Department of Chemical Engineering, National Tsing Hua University, 101, Kuang-Fu Road, 300, Hsin-Chu City, Taiwan aut NATIONALLICENCE 773 0- Cytotechnology Springer Netherlands 63/1(2011-01-01), 13-23 0920-9069 63:1<13 2011 63 10616 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer