caa a22 4500 445822910 CHVBK 20180317145249.0 cr unu---uuuuu 170323e20110101xx s 000 0 eng 10.1007/s10616-010-9325-6 doi (NATIONALLICENCE)springer-10.1007/s10616-010-9325-6 A test facility for fritted spargers of production-scale-bioreactors [Elektronische Daten] [C. Sieblist, M. Aehle, M. Pohlscheidt, M. Jenzsch, A. Lübbert] The production of therapeutic proteins requires qualification of equipment components and appropriate validation procedures for all operations. Since protein productions are typically performed in bioreactors using aerobic cultivation processes air sparging is an essential factor. As recorded in literature, besides ring spargers and open pipe, sinter frits are often used as sparging elements in large scale bioreactors. Due to the manufacturing process these frits have a high lot-to-lot product variability. Experience shows this is a practical problem for use in production processes of therapeutic proteins, hence frits must be tested before they can be employed. The circumstance of checking quality and performance of frits as sparging elements was investigated and various possibilities have been compared. Criteria have been developed in order to evaluate the sparging performance under conditions comparable to those in production bioreactors. The oxygen mass transfer coefficient (k L a) was chosen as the evaluation criterion. It is well known as an essential performance measure for fermenters in the monoclonal antibody production. Therefore a test rig was constructed able to automatically test frit-spargers with respect to their k L a-values at various gas throughputs. Performance differences in the percent range could be detected. Springer Science+Business Media B.V., 2010 Fritted spargers nationallicence Bubble aeration nationallicence Oxygen supply nationallicence Animal cell bioreactors nationallicence Sieblist C. Institute of Biochemistry and Biotechnology, Center for Bioprocess Engineering, Martin-Luther-University Halle-Wittenberg, Kurt-Mothes-Str. 3 / 320.2, 06120, Halle, Saale, Germany aut Aehle M. Institute of Biochemistry and Biotechnology, Center for Bioprocess Engineering, Martin-Luther-University Halle-Wittenberg, Kurt-Mothes-Str. 3 / 320.2, 06120, Halle, Saale, Germany aut Pohlscheidt M. Roche Diagnostics GmbH, Nonnenwald 2, 82377, Penzberg, Germany aut Jenzsch M. Roche Diagnostics GmbH, Nonnenwald 2, 82377, Penzberg, Germany aut Lübbert A. Institute of Biochemistry and Biotechnology, Center for Bioprocess Engineering, Martin-Luther-University Halle-Wittenberg, Kurt-Mothes-Str. 3 / 320.2, 06120, Halle, Saale, Germany aut Cytotechnology Springer Netherlands 63/1(2011-01-01), 49-55 0920-9069 63:1<49 2011 63 10616 https://doi.org/10.1007/s10616-010-9325-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10616-010-9325-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sieblist C. Institute of Biochemistry and Biotechnology, Center for Bioprocess Engineering, Martin-Luther-University Halle-Wittenberg, Kurt-Mothes-Str. 3 / 320.2, 06120, Halle, Saale, Germany aut NATIONALLICENCE 700 1- Aehle M. Institute of Biochemistry and Biotechnology, Center for Bioprocess Engineering, Martin-Luther-University Halle-Wittenberg, Kurt-Mothes-Str. 3 / 320.2, 06120, Halle, Saale, Germany aut NATIONALLICENCE 700 1- Pohlscheidt M. Roche Diagnostics GmbH, Nonnenwald 2, 82377, Penzberg, Germany aut NATIONALLICENCE 700 1- Jenzsch M. Roche Diagnostics GmbH, Nonnenwald 2, 82377, Penzberg, Germany aut NATIONALLICENCE 700 1- Lübbert A. Institute of Biochemistry and Biotechnology, Center for Bioprocess Engineering, Martin-Luther-University Halle-Wittenberg, Kurt-Mothes-Str. 3 / 320.2, 06120, Halle, Saale, Germany aut NATIONALLICENCE 773 0- Cytotechnology Springer Netherlands 63/1(2011-01-01), 49-55 0920-9069 63:1<49 2011 63 10616 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer