caa a22 4500 445822929 CHVBK 20180317145250.0 cr unu---uuuuu 170323e20110101xx s 000 0 eng 10.1007/s10616-010-9327-4 doi (NATIONALLICENCE)springer-10.1007/s10616-010-9327-4 Transforming growth factor-beta 1 in adipose derived stem cells conditioned medium is a dominant paracrine mediator determines hyaluronic acid and collagen expression profile [Elektronische Daten] [Hana Jung, Hak Kim, Dong Lee, Yu-Shik Hwang, Hyeong-Cheol Yang, Jong-Chul Park] Conditioned medium from adipose derived stem cells (ADSC-CM) stimulates both collagen synthesis and migration of fibroblasts, and accelerates wound healing in vivo. Recently, the production and secretion of growth factors has been identified as an essential function of adipose-derived stem cells (ADSCs). However, the main soluble factor of ADSC-CM which mediates paracrine effects and its underlying mechanism has not been elucidated yet. In this study, we considered transforming growth factor-beta1 (TGF-β1) as a strong candidate for paracrine effect of ADSC-CM and investigated collagen synthesis and hyaluronic acid synthase (HAS) expression. After ADSC-CM addition, collagen type I, type III, HAS and hyaluronic acid (HA) expressions on human dermal fibroblasts (HDFs) were evaluated. Furthermore, to clarify effects of TGF-β1 as a paracrine mediator, TGF-β1 antibody and external supplementary TGF-β1 were treated to HDFs. Collagens type I, type III, HAS-1 and HAS-2 mRNA expressions of HDFs were greatly increased by ADSC-CM treatment, however there was no change in TGF-β1 antibody treated HDFs compared with non-treated control. These results strongly demonstrate that TGF-β1 plays an important role as a paracrine mediator of ECM synthesis. The fact that TGF-β1 contained in ADSC-CM not only accelerates collagen deposition but also increase hyaluronic acid synthesis of HDFs through HAS-1 and HAS-2 expression was also elucidated in this study. Therefore, ADSC-CM shows promise for the treatment of cutaneous wounds and accelerates granulation formation during healing process. Springer Science+Business Media B.V., 2010 Adipose derived stem cells (ADSCs) nationallicence Paracrine effect nationallicence TGF-β1 nationallicence Hyaluronic acid synthase (HAS) nationallicence Human dermal fibroblasts (HDFs) nationallicence Jung Hana Biomaterials Research Center, Cellinbio, 103-1208 DigitalEmpire II, 486, Sindong, 443-734, Yeongtong-gu, Suwon-si, Gyeonggi-do, Korea aut Kim Hak Department of Medical Engineering, Yonsei University College of Medicine, 134 Shinchon-dong, Seodaemun-gu, 120-752, Seoul, Korea aut Lee Dong Biomaterials Research Center, Cellinbio, 103-1208 DigitalEmpire II, 486, Sindong, 443-734, Yeongtong-gu, Suwon-si, Gyeonggi-do, Korea aut Hwang Yu-Shik Department of Maxillofacial Biomedical Engineering and Institute of Oral Biology School of Dentistry, Kyung Hee University, 1 Hoegi-dong, Dongdaemun-ku, 130-701, Seoul, Korea aut Yang Hyeong-Cheol Department of Dental Biomaterials Science and Dental Research Institute, College of Dentistry, Seoul National University, 28 Yeonkun-dong, Chongro-ku, 110-749, Seoul, Korea aut Park Jong-Chul Department of Medical Engineering, Yonsei University College of Medicine, 134 Shinchon-dong, Seodaemun-gu, 120-752, Seoul, Korea aut Cytotechnology Springer Netherlands 63/1(2011-01-01), 57-66 0920-9069 63:1<57 2011 63 10616 https://doi.org/10.1007/s10616-010-9327-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10616-010-9327-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jung Hana Biomaterials Research Center, Cellinbio, 103-1208 DigitalEmpire II, 486, Sindong, 443-734, Yeongtong-gu, Suwon-si, Gyeonggi-do, Korea aut NATIONALLICENCE 700 1- Kim Hak Department of Medical Engineering, Yonsei University College of Medicine, 134 Shinchon-dong, Seodaemun-gu, 120-752, Seoul, Korea aut NATIONALLICENCE 700 1- Lee Dong Biomaterials Research Center, Cellinbio, 103-1208 DigitalEmpire II, 486, Sindong, 443-734, Yeongtong-gu, Suwon-si, Gyeonggi-do, Korea aut NATIONALLICENCE 700 1- Hwang Yu-Shik Department of Maxillofacial Biomedical Engineering and Institute of Oral Biology School of Dentistry, Kyung Hee University, 1 Hoegi-dong, Dongdaemun-ku, 130-701, Seoul, Korea aut NATIONALLICENCE 700 1- Yang Hyeong-Cheol Department of Dental Biomaterials Science and Dental Research Institute, College of Dentistry, Seoul National University, 28 Yeonkun-dong, Chongro-ku, 110-749, Seoul, Korea aut NATIONALLICENCE 700 1- Park Jong-Chul Department of Medical Engineering, Yonsei University College of Medicine, 134 Shinchon-dong, Seodaemun-gu, 120-752, Seoul, Korea aut NATIONALLICENCE 773 0- Cytotechnology Springer Netherlands 63/1(2011-01-01), 57-66 0920-9069 63:1<57 2011 63 10616 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer