caa a22 4500 445822937 CHVBK 20180317145250.0 cr unu---uuuuu 170323e20110101xx s 000 0 eng 10.1007/s10616-010-9324-7 doi (NATIONALLICENCE)springer-10.1007/s10616-010-9324-7 Comparison of tetrahydrofuran, fetal calf serum, and Tween 40 for the delivery of astaxanthin and canthaxanthin to HepG2 cells [Elektronische Daten] [Christine Boesch-Saadatmandi, Gerald Rimbach, Alexander Jungblut, Jan Frank] The present investigation aimed to compare fetal calf serum (FCS) and Tween 40 with the commonly employed tetrahydrofuran (THF) with respect to cytotoxicity, stability of the solubilized carotenoids, and uptake and accumulation of the xanthophylls astaxanthin (AX) and canthaxanthin (CX) in cultured human liver cells (HepG2). Incubation of HepG2 cells for 24h with THF (≥1.25%) or FCS (≥11.25%) with or without AX (≥25μmol/L) or CX (≥25μmol/L) did not affect cell viability. Tween 40 (0.25-1.25% in medium) reduced cell viability by 75-99%. The stabilities of AX and CX in cell-free RPMI 1640 medium for ≤24h were higher when delivered with THF instead of FCS. The dose- and time-dependent accumulations of AX and CX (1-10μmol/L) in HepG2 cells were higher when carotenoids were delivered with FCS compared to THF. In conclusion, FCS and THF, but not Tween 40, were suitable solvent systems for the delivery of AX and CX to HepG2 cells. In our experiments FCS was superior with regard to the uptake and accumulation of both carotenoids. Springer Science+Business Media B.V., 2010 Carotenoids nationallicence Astaxanthin nationallicence Canthaxanthin nationallicence Delivery vehicle nationallicence HepG2 cell culture nationallicence Fetal calf serum nationallicence Tween 40 nationallicence Tetrahydrofuran nationallicence Boesch-Saadatmandi Christine Institute of Human Nutrition and Food Science, Christian-Albrechts-University Kiel, Hermann-Rodewald-Str. 6, 24118, Kiel, Germany aut Rimbach Gerald Institute of Human Nutrition and Food Science, Christian-Albrechts-University Kiel, Hermann-Rodewald-Str. 6, 24118, Kiel, Germany aut Jungblut Alexander Institute of Human Nutrition and Food Science, Christian-Albrechts-University Kiel, Hermann-Rodewald-Str. 6, 24118, Kiel, Germany aut Frank Jan Institute of Biological Chemistry and Nutrition (140f), University of Hohenheim, Garbenstr. 28, 70599, Stuttgart, Germany aut Cytotechnology Springer Netherlands 63/1(2011-01-01), 89-97 0920-9069 63:1<89 2011 63 10616 https://doi.org/10.1007/s10616-010-9324-7 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10616-010-9324-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Boesch-Saadatmandi Christine Institute of Human Nutrition and Food Science, Christian-Albrechts-University Kiel, Hermann-Rodewald-Str. 6, 24118, Kiel, Germany aut NATIONALLICENCE 700 1- Rimbach Gerald Institute of Human Nutrition and Food Science, Christian-Albrechts-University Kiel, Hermann-Rodewald-Str. 6, 24118, Kiel, Germany aut NATIONALLICENCE 700 1- Jungblut Alexander Institute of Human Nutrition and Food Science, Christian-Albrechts-University Kiel, Hermann-Rodewald-Str. 6, 24118, Kiel, Germany aut NATIONALLICENCE 700 1- Frank Jan Institute of Biological Chemistry and Nutrition (140f), University of Hohenheim, Garbenstr. 28, 70599, Stuttgart, Germany aut NATIONALLICENCE 773 0- Cytotechnology Springer Netherlands 63/1(2011-01-01), 89-97 0920-9069 63:1<89 2011 63 10616 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer