caa a22 4500 445822945 CHVBK 20180317145250.0 cr unu---uuuuu 170323e20110101xx s 000 0 eng 10.1007/s10616-010-9328-3 doi (NATIONALLICENCE)springer-10.1007/s10616-010-9328-3 Long-term cultures of stem/progenitor cells from lobular and ductal breast carcinomas under non-adherent conditions [Elektronische Daten] [Agostina Nardone, Sara Corvigno, Annalisa Brescia, Daniel D'Andrea, Gennaro Limite, Bianca Veneziani] A small subpopulation of stem/progenitor cells can give rise to the diversity of differentiated cells that comprise the bulk of the tumor. Are proliferating cells, within the bulk of tumor, few cells with uncommon features? The cell biological approach provides a limitless model for studying the hierarchical organization of progenitor subpopulation and identifying potential therapeutic targets. Aim of the study was to expand patients' breast cancer cells for evaluating functional cell properties, and to characterize the protein expression profile of selected cells to be compared with that of primary tumors. Breast cancer cells from estrogen receptor (ERα) positive, HER2 negative lobular (LoBS cells) and ductal (DuBS cells) histotype were cultured under non-adherent conditions to form mammospheres. Sorting of the cells by their surface expression of CD24 and CD44 gave rise to subpopulations which were propagated, enriched and characterized for the expression of epithelial and stromal markers. We found that non-adherent culture conditions generate mammospheres of slowly proliferating cells; single cells, dissociated from mammospheres, grow in soft agar; long-term cultured LoBS and DuBS cells, CD44+/CD24low, express cytokeratin 5 (CK5), α-smooth muscle actin (α-sma) and vimentin, known as markers of basal/myoepithelial cells; and ERα (only DuBS cells), HER1 (EGF-Receptor), activated HER2, and cyclinD1 as markers of luminal epithelial cell. Isolates of cells from breast cancer patients may be a tool for a marker-driven testing of targeted therapies. Springer Science+Business Media B.V., 2010 Breast cancer therapy nationallicence Epithelial stromal cells nationallicence Nardone Agostina Dipartimento di Biologia e Patologia Cellulare e Molecolare "L. Califano”, Università di Napoli Federico II, via Pansini 5, 80131, Naples, Italy aut Corvigno Sara Dipartimento di Biologia e Patologia Cellulare e Molecolare "L. Califano”, Università di Napoli Federico II, via Pansini 5, 80131, Naples, Italy aut Brescia Annalisa Dipartimento di Biologia e Patologia Cellulare e Molecolare "L. Califano”, Università di Napoli Federico II, via Pansini 5, 80131, Naples, Italy aut D'Andrea Daniel Dipartimento di Biologia e Patologia Cellulare e Molecolare "L. Califano”, Università di Napoli Federico II, via Pansini 5, 80131, Naples, Italy aut Limite Gennaro Dipartimento di Chirurgia Generale Oncologica e Tecnologie Avanzate, Università degli Studi di Napoli Federico II, via Pansini 5, 80131, Naples, Italy aut Veneziani Bianca Dipartimento di Biologia e Patologia Cellulare e Molecolare "L. Califano”, Università di Napoli Federico II, via Pansini 5, 80131, Naples, Italy aut Cytotechnology Springer Netherlands 63/1(2011-01-01), 67-80 0920-9069 63:1<67 2011 63 10616 https://doi.org/10.1007/s10616-010-9328-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10616-010-9328-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nardone Agostina Dipartimento di Biologia e Patologia Cellulare e Molecolare "L. Califano”, Università di Napoli Federico II, via Pansini 5, 80131, Naples, Italy aut NATIONALLICENCE 700 1- Corvigno Sara Dipartimento di Biologia e Patologia Cellulare e Molecolare "L. Califano”, Università di Napoli Federico II, via Pansini 5, 80131, Naples, Italy aut NATIONALLICENCE 700 1- Brescia Annalisa Dipartimento di Biologia e Patologia Cellulare e Molecolare "L. Califano”, Università di Napoli Federico II, via Pansini 5, 80131, Naples, Italy aut NATIONALLICENCE 700 1- D'Andrea Daniel Dipartimento di Biologia e Patologia Cellulare e Molecolare "L. Califano”, Università di Napoli Federico II, via Pansini 5, 80131, Naples, Italy aut NATIONALLICENCE 700 1- Limite Gennaro Dipartimento di Chirurgia Generale Oncologica e Tecnologie Avanzate, Università degli Studi di Napoli Federico II, via Pansini 5, 80131, Naples, Italy aut NATIONALLICENCE 700 1- Veneziani Bianca Dipartimento di Biologia e Patologia Cellulare e Molecolare "L. Califano”, Università di Napoli Federico II, via Pansini 5, 80131, Naples, Italy aut NATIONALLICENCE 773 0- Cytotechnology Springer Netherlands 63/1(2011-01-01), 67-80 0920-9069 63:1<67 2011 63 10616 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer