caa a22 4500 445822953 CHVBK 20180317145250.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s10616-011-9380-7 doi (NATIONALLICENCE)springer-10.1007/s10616-011-9380-7 Derivation, characterization and expansion of fetal chondrocytes on different microcarriers [Elektronische Daten] [Gaye Çetinkaya, Anıl Kahraman, Menemşe Gümüşderelioğlu, Sezen Arat, Mehmet Onur] Fetal chondrocytes (FCs) have recently been identified as an alternative cell source for cartilage tissue engineering applications because of their partially chondrogenically differentiated phenotype and developmental plasticity. In this study, chondrocytes derived from fetal bovine cartilage were characterized and then cultured on commercially available Cytodex-1 and Biosilon microcarriers and thermosensitive poly(hydroxyethylmethacrylate)-poly(N-isopropylacrylamide) (PHEMA-PNIPAAm) beads produced by us. Growth kinetics of FCs were estimated by means of specific growth rate and metabolic activity assay. Cell detachment from thermosensitive microcarriers was induced by cold treatment at 4°C for 20min or enzymatic treatment was applied for the detachment of cells from Cytodex-1 and Biosilon. Although attachment efficiency and proliferation of FCs on PHEMA-PNIPAAm beads were lower than that of commercial Cytodex-1 and Biosilon microcarriers, these beads also supported growth of FCs. Detached cells from thermosensitive beads by cold induction exhibited a normal proliferative activity. Our results indicated that Cytodex-1 microcarrier was the most suitable material for the production of FCs in high capacity, however, ‘thermosensitive microcarrier model' could be considered as an attractive solution to the process scale up for cartilage tissue engineering by improving surface characteristics of PHEMA-PNIPAAm beads. Springer Science+Business Media B.V., 2011 Fetal chondrocyte nationallicence Thermosensitive polymers nationallicence Microcarriers nationallicence PNIPAAm nationallicence Cartilage tissue engineering nationallicence Çetinkaya Gaye TUBITAK MRC-Genetic Engineering Biotechnology Institute (GEBI), 41470, Gebze/Kocaeli, Turkey aut Kahraman Anıl Chemical Engineering and Bioengineering Departments, Hacettepe University, 06800, Beytepe, Ankara, Turkey aut Gümüşderelioğlu Menemşe Chemical Engineering and Bioengineering Departments, Hacettepe University, 06800, Beytepe, Ankara, Turkey aut Arat Sezen TUBITAK MRC-Genetic Engineering Biotechnology Institute (GEBI), 41470, Gebze/Kocaeli, Turkey aut Onur Mehmet Faculty of Science, Department of Biology, Hacettepe University, 06800, Beytepe, Ankara, Turkey aut Cytotechnology Springer Netherlands 63/6(2011-12-01), 633-643 0920-9069 63:6<633 2011 63 10616 https://doi.org/10.1007/s10616-011-9380-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10616-011-9380-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Çetinkaya Gaye TUBITAK MRC-Genetic Engineering Biotechnology Institute (GEBI), 41470, Gebze/Kocaeli, Turkey aut NATIONALLICENCE 700 1- Kahraman Anıl Chemical Engineering and Bioengineering Departments, Hacettepe University, 06800, Beytepe, Ankara, Turkey aut NATIONALLICENCE 700 1- Gümüşderelioğlu Menemşe Chemical Engineering and Bioengineering Departments, Hacettepe University, 06800, Beytepe, Ankara, Turkey aut NATIONALLICENCE 700 1- Arat Sezen TUBITAK MRC-Genetic Engineering Biotechnology Institute (GEBI), 41470, Gebze/Kocaeli, Turkey aut NATIONALLICENCE 700 1- Onur Mehmet Faculty of Science, Department of Biology, Hacettepe University, 06800, Beytepe, Ankara, Turkey aut NATIONALLICENCE 773 0- Cytotechnology Springer Netherlands 63/6(2011-12-01), 633-643 0920-9069 63:6<633 2011 63 10616 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer