caa a22 4500 445822961 CHVBK 20180317145250.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s10616-011-9378-1 doi (NATIONALLICENCE)springer-10.1007/s10616-011-9378-1 Efficiency of Plasmocin™ on various mammalian cell lines infected by mollicutes in comparison with commonly used antibiotics in cell culture: a local experience [Elektronische Daten] [Vahid Molla Kazemiha, Shahram Azari, Amir Amanzadeh, Shahin Bonakdar, Morteza Shojaei Moghadam, Mahdi Habibi Anbouhi, Susan Maleki, Nahid Ahmadi, Tahmineh Mousavi, Mohammad Shokrgozar] Mycoplasma contamination is a deleterious event for cell culture laboratories. Plasmocin™ is used to prevent and eradicate mycoplasma infections from cell. In this study, 80 different mammalian cell lines from various sources; human, monkey, mice, hamster and rat were used to study and evaluate plasmocin™ efficiency and compare it to commonly used antibiotics such as BM-cyclin, ciprofloxacin and mycoplasma removal agent (MRA). It was shown that mycoplasma infections were eradicated by plasmocin™, BM-cyclin, ciprofloxacin and MRA in 65%, 66.25%, 20%, and 31.25%, respectively, of infected cell cultures. However, re-infection with mycoplasmas after the period of 4months occurred in 10-80% of the studied cell lines. Cell cytotoxicity and culture death was observed in 25, 17.5 and 10% of the treated cells, for plasmocin™, BM-cyclin and MRA, respectively. In this study, Plasmocin™ showed strong ability to eradicate mollicutes from our cell lines with minimal percentage of regrowth. However, due to its high cell cytotoxicity it should be used with caution especially when dealing with expensive or hard-to-obtain cell lines. Amongst the antibiotics tested, BM-cyclin was shown to remove mycoplasma with the highest efficiency. Springer Science+Business Media B.V., 2011 Mycoplasma nationallicence Plasmocin nationallicence Treatment nationallicence Cell line nationallicence Cytotoxicity nationallicence Molla Kazemiha Vahid National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran aut Azari Shahram National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran aut Amanzadeh Amir National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran aut Bonakdar Shahin National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran aut Shojaei Moghadam Morteza Department of Biotechnology, Norwegian University of Science and Technology, Trondheim, Norway aut Habibi Anbouhi Mahdi National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran aut Maleki Susan Department of Biotechnology, Norwegian University of Science and Technology, Trondheim, Norway aut Ahmadi Nahid National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran aut Mousavi Tahmineh National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran aut Shokrgozar Mohammad National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran aut Cytotechnology Springer Netherlands 63/6(2011-12-01), 609-620 0920-9069 63:6<609 2011 63 10616 https://doi.org/10.1007/s10616-011-9378-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10616-011-9378-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Molla Kazemiha Vahid National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran aut NATIONALLICENCE 700 1- Azari Shahram National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran aut NATIONALLICENCE 700 1- Amanzadeh Amir National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran aut NATIONALLICENCE 700 1- Bonakdar Shahin National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran aut NATIONALLICENCE 700 1- Shojaei Moghadam Morteza Department of Biotechnology, Norwegian University of Science and Technology, Trondheim, Norway aut NATIONALLICENCE 700 1- Habibi Anbouhi Mahdi National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran aut NATIONALLICENCE 700 1- Maleki Susan Department of Biotechnology, Norwegian University of Science and Technology, Trondheim, Norway aut NATIONALLICENCE 700 1- Ahmadi Nahid National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran aut NATIONALLICENCE 700 1- Mousavi Tahmineh National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran aut NATIONALLICENCE 700 1- Shokrgozar Mohammad National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran aut NATIONALLICENCE 773 0- Cytotechnology Springer Netherlands 63/6(2011-12-01), 609-620 0920-9069 63:6<609 2011 63 10616 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer