caa a22 4500 445822996 CHVBK 20180317145250.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s10616-011-9384-3 doi (NATIONALLICENCE)springer-10.1007/s10616-011-9384-3 In vitro cytokine stimulation assay for glycolipid biosurfactant from Rhodococcus ruber [Elektronische Daten] role of monocyte adhesion [Sergey Gein, Maria Kuyukina, Irena Ivshina, Tatiana Baeva, Valery Chereshnev] Glycolipid biosurfactant (GLB) from Rhodococcus ruber IEGM 231 was found to stimulate tumor necrosis factor-α (TNF-α), interleukin (IL) -1β and IL-6 production when applied as an ultrasonic emulsion to the adherent human peripheral blood monocyte culture. However, a lack of cytokine-stimulating activity was registered with the GLB applied as a hydrophobic film coating in 24-well culture plates, indicating that it may have been due to its inhibitory effect on monocyte adhesion. The mode of GLB application may therefore play an important role in in vitro assay of immunostimulatory activity of this compound as well as other bacterial glycolipids. Additionally, GLB from R. ruber displayed no cytotoxicity against human lymphocytes and therefore could be proposed as a potential immunomodulating and antitumor agent. Springer Science+Business Media B.V., 2011 Glycolipid biosurfactant nationallicence Rhodococcus nationallicence Cytokine-stimulating activity nationallicence Monocyte adhesion nationallicence Toxicity nationallicence GLB : Glycolipid biosurfactant nationallicence TNF : Tumor necrosis factor nationallicence IL : Interleukin nationallicence TDM : Trehalose-6,6'-dimycolate nationallicence IEGM : Institute of Ecology and Genetics of Microorganisms nationallicence ELISA : Enzyme-linked immunosorbent assay nationallicence LPS : Lipopolysaccharide nationallicence Gein Sergey Institute of Ecology and Genetics of Microorganisms, Ural Branch of the Russian Academy of Sciences, 13 Golev Street, 614081, Perm, Russia aut Kuyukina Maria Institute of Ecology and Genetics of Microorganisms, Ural Branch of the Russian Academy of Sciences, 13 Golev Street, 614081, Perm, Russia aut Ivshina Irena Institute of Ecology and Genetics of Microorganisms, Ural Branch of the Russian Academy of Sciences, 13 Golev Street, 614081, Perm, Russia aut Baeva Tatiana Institute of Ecology and Genetics of Microorganisms, Ural Branch of the Russian Academy of Sciences, 13 Golev Street, 614081, Perm, Russia aut Chereshnev Valery Institute of Ecology and Genetics of Microorganisms, Ural Branch of the Russian Academy of Sciences, 13 Golev Street, 614081, Perm, Russia aut Cytotechnology Springer Netherlands 63/6(2011-12-01), 559-566 0920-9069 63:6<559 2011 63 10616 https://doi.org/10.1007/s10616-011-9384-3 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10616-011-9384-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gein Sergey Institute of Ecology and Genetics of Microorganisms, Ural Branch of the Russian Academy of Sciences, 13 Golev Street, 614081, Perm, Russia aut NATIONALLICENCE 700 1- Kuyukina Maria Institute of Ecology and Genetics of Microorganisms, Ural Branch of the Russian Academy of Sciences, 13 Golev Street, 614081, Perm, Russia aut NATIONALLICENCE 700 1- Ivshina Irena Institute of Ecology and Genetics of Microorganisms, Ural Branch of the Russian Academy of Sciences, 13 Golev Street, 614081, Perm, Russia aut NATIONALLICENCE 700 1- Baeva Tatiana Institute of Ecology and Genetics of Microorganisms, Ural Branch of the Russian Academy of Sciences, 13 Golev Street, 614081, Perm, Russia aut NATIONALLICENCE 700 1- Chereshnev Valery Institute of Ecology and Genetics of Microorganisms, Ural Branch of the Russian Academy of Sciences, 13 Golev Street, 614081, Perm, Russia aut NATIONALLICENCE 773 0- Cytotechnology Springer Netherlands 63/6(2011-12-01), 559-566 0920-9069 63:6<559 2011 63 10616 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer