caa a22 4500 445823003 CHVBK 20180317145250.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s10616-011-9364-7 doi (NATIONALLICENCE)springer-10.1007/s10616-011-9364-7 Transition of basic protein during spermatogenesis of Fenneropenaeus chinensis (Osbeck, 1765) [Elektronische Daten] [Shaoqin Ge, Suixin Wang, Xianjiang Kang, Fei Duan, Yan Wang, Wenyan Li, Mingshen Guo, Shumei Mu, Yuhua Zhang] According to the ultrastructural characteristic observation of the developing male germ cells, spermatogenesis of the crustacean shrimp, Fenneropenaeus chinensis, is classified into spermatogonia, primary spermatocytes, secondary spermatocyte, four stages of spermatids, and mature sperm. The basic protein transition during its spermatogenesis is studied by transmission electron microscopy of ammoniacal silver reaction and immunoelectron microscopical distribution of acetylated histone H4. The results show that basic protein synthesized in cytoplasm of spermatogonia is transferred into the nucleus with deposition on new duplicated DNA. In the spermatocyte stage, some nuclear basic protein combined with RNP is transferred into the cytoplasm and is involved in forming the cytoplasmic vesicle clumps. In the early spermatid, most of the basic protein synthesized in the new spermatid cytoplasm is transferred into the nucleus, and the chromatin condensed gradually, and the rest is shifted into the pre-acrosomal vacuole. In the middle spermatid, the nuclear basic protein linked with DNA is acetylated and transferred into the proacrosomal vacuole and assembled into the acrosomal blastema. At the late spermatid, almost all of the basic protein in the nucleus has been removed into the acrosome. During the stage from late spermatid to mature sperm, some de novo basic proteins synthesized in the cytoplasm belt transfer into the nucleus without a membrane and almost all deposit in the periphery to form a supercoating. The remnant histone H4 accompanied by chromatin fibers is acetylated in the center of the nucleus, leading to relaxed DNA and activated genes making the nucleus non-condensed. Springer Science+Business Media B.V., 2011 Fenneropenaeus chinensis nationallicence Spermatogenesis nationallicence Ultrastructural characteristics nationallicence Basic protein transition nationallicence Acetylated H4 nationallicence Ammoniacal silver reaction nationallicence Immunolocalization nationallicence Ge Shaoqin Hebei University Health Science Center, 071000, Baoding, People's Republic of China aut Wang Suixin Langfang Health Vocational College, 065000, Langfang, People's Republic of China aut Kang Xianjiang College of Life Science, Hebei University, 071000, Baoding, People's Republic of China aut Duan Fei Hebei University Health Science Center, 071000, Baoding, People's Republic of China aut Wang Yan Hebei University Health Science Center, 071000, Baoding, People's Republic of China aut Li Wenyan Hebei University Health Science Center, 071000, Baoding, People's Republic of China aut Guo Mingshen College of Life Science, Hebei University, 071000, Baoding, People's Republic of China aut Mu Shumei College of Life Science, Hebei University, 071000, Baoding, People's Republic of China aut Zhang Yuhua Hebei University Health Science Center, 071000, Baoding, People's Republic of China aut Cytotechnology Springer Netherlands 63/6(2011-12-01), 581-598 0920-9069 63:6<581 2011 63 10616 https://doi.org/10.1007/s10616-011-9364-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10616-011-9364-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ge Shaoqin Hebei University Health Science Center, 071000, Baoding, People's Republic of China aut NATIONALLICENCE 700 1- Wang Suixin Langfang Health Vocational College, 065000, Langfang, People's Republic of China aut NATIONALLICENCE 700 1- Kang Xianjiang College of Life Science, Hebei University, 071000, Baoding, People's Republic of China aut NATIONALLICENCE 700 1- Duan Fei Hebei University Health Science Center, 071000, Baoding, People's Republic of China aut NATIONALLICENCE 700 1- Wang Yan Hebei University Health Science Center, 071000, Baoding, People's Republic of China aut NATIONALLICENCE 700 1- Li Wenyan Hebei University Health Science Center, 071000, Baoding, People's Republic of China aut NATIONALLICENCE 700 1- Guo Mingshen College of Life Science, Hebei University, 071000, Baoding, People's Republic of China aut NATIONALLICENCE 700 1- Mu Shumei College of Life Science, Hebei University, 071000, Baoding, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Yuhua Hebei University Health Science Center, 071000, Baoding, People's Republic of China aut NATIONALLICENCE 773 0- Cytotechnology Springer Netherlands 63/6(2011-12-01), 581-598 0920-9069 63:6<581 2011 63 10616 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer