caa a22 4500 445823011 CHVBK 20180317145250.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s10616-011-9391-4 doi (NATIONALLICENCE)springer-10.1007/s10616-011-9391-4 Cytocentrifugation: a convenient and efficient method for seeding tendon-derived cells into monolayer cultures or 3-D tissue engineering scaffolds [Elektronische Daten] [Louise Way, Nanette Scutt, Andrew Scutt] Tendon and ligament injuries are very common, requiring some 200,000 reconstructions per year in the USA. Autografting can be used to repair these but donor tissue is limited and harvesting leads to morbidity at the graft sites. Tissue engineering has been used to grow simple tissues such as skin, cartilage and bone and due to their low vascularity and simple structure, tendons should be ideal candidates for such an approach. Scaffolds are essential for tissue engineering as they provide structure and signals that regulate growth. However, they present a physical barrier to cell seeding with the majority of the cells congregating at the scaffold surface. To address this we used centrifugation to enhance penetration of tendon-derived cells to the centres of 3-D scaffolds. The process had no apparent deleterious effects on the cells and both plating efficiency and cell distribution improved. After attachment the cells continued to proliferate and deposit a collagenous matrix. Scaffold penetration was investigated using layers of Azowipes allowing the separation and examination of individual leaves. At relatively low g-forces, cells penetrated a stack of 6 Azowipes leaving cells attached to each leaf. These data suggest that cytocentrifugation improves the penetration and homogeneity of tendon derived cells in 3-D and monolayer cultures. Springer Science+Business Media B.V., 2011 Tissue engineering nationallicence Cytocentrifugation nationallicence Scaffold nationallicence Tendon nationallicence Tenocytes nationallicence Cell culture nationallicence Way Louise Bone Biology Group, Department of Human Metabolism, Faculty of Medicine, Dentistry and Health, University of Sheffield, Beech Hill Road, S10 2RX, Sheffield, UK aut Scutt Nanette Bone Biology Group, Department of Human Metabolism, Faculty of Medicine, Dentistry and Health, University of Sheffield, Beech Hill Road, S10 2RX, Sheffield, UK aut Scutt Andrew Bone Biology Group, Department of Human Metabolism, Faculty of Medicine, Dentistry and Health, University of Sheffield, Beech Hill Road, S10 2RX, Sheffield, UK aut Cytotechnology Springer Netherlands 63/6(2011-12-01), 567-579 0920-9069 63:6<567 2011 63 10616 https://doi.org/10.1007/s10616-011-9391-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10616-011-9391-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Way Louise Bone Biology Group, Department of Human Metabolism, Faculty of Medicine, Dentistry and Health, University of Sheffield, Beech Hill Road, S10 2RX, Sheffield, UK aut NATIONALLICENCE 700 1- Scutt Nanette Bone Biology Group, Department of Human Metabolism, Faculty of Medicine, Dentistry and Health, University of Sheffield, Beech Hill Road, S10 2RX, Sheffield, UK aut NATIONALLICENCE 700 1- Scutt Andrew Bone Biology Group, Department of Human Metabolism, Faculty of Medicine, Dentistry and Health, University of Sheffield, Beech Hill Road, S10 2RX, Sheffield, UK aut NATIONALLICENCE 773 0- Cytotechnology Springer Netherlands 63/6(2011-12-01), 567-579 0920-9069 63:6<567 2011 63 10616 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer