caa a22 4500 44582302X CHVBK 20180317145250.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s10616-011-9394-1 doi (NATIONALLICENCE)springer-10.1007/s10616-011-9394-1 Species identification and authentification of human and rodent cell cultures using polymerase chain reaction analysis of vomeronasal receptor genes [Elektronische Daten] [M. Holder, P. Cooper] Cell culture and the use of cell lines are routinely used in basic scientific research. It is therefore imperative for researchers to ensure the origin of the cell lines used and that they are routinely re-analysed for contamination and misidentification. Inter-species contamination is relatively frequent, and the most commonly used cell lines are of human, mouse and rat derivation. We have developed simple species specific primer assays based on genomic sequence differences in vomeronasal receptor gene family members to discriminate between human, mouse and rat DNA using standard agarose gel electrophoresis. Furthermore, these PCR assays are able to identify the species composition within an inter-species mixed population. This approach therefore provides a valuable tool to enable a rapid, simple and relatively inexpensive determination of the authentication and contamination of cell cultures. Springer Science+Business Media B.V., 2011 PCR nationallicence Vomeronasal receptors nationallicence Species specificity nationallicence Genomic DNA nationallicence Holder M. Molecular Biology, School of Dentistry, University of Birmingham, St Chads Queensway, B4 6NN, Birmingham, UK aut Cooper P. Molecular Biology, School of Dentistry, University of Birmingham, St Chads Queensway, B4 6NN, Birmingham, UK aut Cytotechnology Springer Netherlands 63/6(2011-12-01), 553-558 0920-9069 63:6<553 2011 63 10616 https://doi.org/10.1007/s10616-011-9394-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10616-011-9394-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Holder M. Molecular Biology, School of Dentistry, University of Birmingham, St Chads Queensway, B4 6NN, Birmingham, UK aut NATIONALLICENCE 700 1- Cooper P. Molecular Biology, School of Dentistry, University of Birmingham, St Chads Queensway, B4 6NN, Birmingham, UK aut NATIONALLICENCE 773 0- Cytotechnology Springer Netherlands 63/6(2011-12-01), 553-558 0920-9069 63:6<553 2011 63 10616 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer