caa a22 4500 445823038 CHVBK 20180317145250.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s10616-011-9387-0 doi (NATIONALLICENCE)springer-10.1007/s10616-011-9387-0 Adaptation of the Se301 insect cell line to suspension culture. Effect of turbulence on growth and on production of nucleopolyhedrovius (SeMNPV) [Elektronische Daten] [Alba Beas-Catena, Asterio Sánchez-Mirón, Francisco García-Camacho, Emilio Molina-Grima] As chemical pesticides are being banned as control agents for agricultural pests, the use of the highly specific, safe to non-target organisms baculoviruses has been proposed. These viruses can be produced either in vivo or in vitro. In vitro production requires appropriated host insect cell lines with the ability for growing as freely-suspended cells. In this work, the Spodoptera exigua Se301 cell line was used to produce the commercially available S. exigua nucleopolyhedrovirus (SeMNPV) in suspension. Se301 cells showed to be very sensitive to the hydrodynamic shear rates developed in bioreactors. A process of progressive adaptation to freely-suspended cultures using protective additives against shear stress and disaggregant was proposed. The best combinations were polyvinyl alcohol (PVA) or polyvinyl pyrrolidone (PVP) with the disaggregant dextran sulfate (DS). Both static and freely-suspended Se301 cell cultures were successfully infected with the SeMNPV baculovirus. Production of occluded baculovirus (OB) increased with the multiplicity of infection (MOI>0.1). Springer Science+Business Media B.V., 2011 Shear rate nationallicence Insect cell nationallicence Baculovirus nationallicence Damage nationallicence Spodoptera nationallicence Turbulence nationallicence Beas-Catena Alba Department of Chemical Engineering, University of Almería, 04120, Almería, Spain aut Sánchez-Mirón Asterio Department of Chemical Engineering, University of Almería, 04120, Almería, Spain aut García-Camacho Francisco Department of Chemical Engineering, University of Almería, 04120, Almería, Spain aut Molina-Grima Emilio Department of Chemical Engineering, University of Almería, 04120, Almería, Spain aut Cytotechnology Springer Netherlands 63/6(2011-12-01), 543-552 0920-9069 63:6<543 2011 63 10616 https://doi.org/10.1007/s10616-011-9387-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10616-011-9387-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Beas-Catena Alba Department of Chemical Engineering, University of Almería, 04120, Almería, Spain aut NATIONALLICENCE 700 1- Sánchez-Mirón Asterio Department of Chemical Engineering, University of Almería, 04120, Almería, Spain aut NATIONALLICENCE 700 1- García-Camacho Francisco Department of Chemical Engineering, University of Almería, 04120, Almería, Spain aut NATIONALLICENCE 700 1- Molina-Grima Emilio Department of Chemical Engineering, University of Almería, 04120, Almería, Spain aut NATIONALLICENCE 773 0- Cytotechnology Springer Netherlands 63/6(2011-12-01), 543-552 0920-9069 63:6<543 2011 63 10616 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer