caa a22 4500 445823062 CHVBK 20180317145250.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s10616-011-9381-6 doi (NATIONALLICENCE)springer-10.1007/s10616-011-9381-6 Crude polysaccharide from an anti-UVB cell clone of Bupleurum scorzonerifolium protect HaCaT cells against UVB-induced oxidative stress [Elektronische Daten] [Jinran Dai, Haiyin Ma, Jing Fan, Yuzhong Li, Jianguang Wang, Hongmei Ni, Guangmin Xia, Suiyun Chen] Bupleurum scorzonerifolium Willd has been found to have a wide range of immunopharmacologic functions. We isolated an anti-UVB B. scorzonerifolium cell clone and found elevated level of polysaccharides. In this study, we investigated the ability of crude polysaccharide (CP) from the anti-UVB B. scorzonerifolium cell clone to inhibit UVB-induced photodamage using a human skin keratinocyte cell line, HaCaT. Cells were UVB irradiated and then incubated in presence of different concentrations of CP. MTT assay showed that the CP did not induce cytotoxic effect under 10mg/mL and after UVB irradiation, CP can inhibit UVB-induced HaCaT cell death. Decreased reactive oxygen species and lipid peroxidation and increased superoxide dismutase activity showed that CP can act as a free radical scavenger. Furthermore, CP had a strong protective ability against UVB-induced DNA damage. These effects were compared to the crude polysaccharide (CP′) from normal B. scorzonerifolium callus at concentration of 20mg/mL. The portion of crude polysaccharide (CP) from the anti-UVB B. scorzonerifolium cell clone was more than 2.5-fold higher than crude polysaccharide (CP′) from normal B. scorzonerifolium callus. Taken together, the protective mechanisms of crude polysaccharide from the anti-UVB B. scorzonerifolium cell clone against UVB-induced photodamage occur by the inhibition of UVB-induced reactive oxygen species production, lipid peroxidation and DNA damage. Springer Science+Business Media B.V., 2011 Anti-UVB Bupleurum scorzonerifolium cell clone nationallicence Crude polysaccharide nationallicence Oxidative stress nationallicence Photodamage nationallicence UVB nationallicence 2,4-D : 2,4-dichlorophenoxyaceic acid nationallicence CM-DCF : Chloromethyl 2′,7′-dichlorodihydrofluorescein diacetate nationallicence CM-H2DCFDA : 5-(and-6)-chloromethyl-2′,7′-dichlorodihydrofluorescein diacetate acetyl ester nationallicence CP : Crude polysaccharides from anti-UVB B. scorzonerifolium cell clone nationallicence CP′ : Crude polysaccharides from normal callus of B. scorzonerifolium nationallicence CP* : Crude polysaccharides from the aerial parts of Wild B. scorzonerifolium nationallicence DMEM : Dulbecco's modified Eagle medium nationallicence DMSO : Dimethylsulfoxide nationallicence FCS : Fetal calf serum nationallicence MDA : Malondialdehyde nationallicence MTT : 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide nationallicence ROS : Reactive oxygen species nationallicence SOD : Superoxide dismutase nationallicence UVB : Ultraviolet-B nationallicence Dai Jinran Plant Science Institute, School of Life Sciences, Yunnan University, 650091, Kunming, China aut Ma Haiyin Plant Science Institute, School of Life Sciences, Yunnan University, 650091, Kunming, China aut Fan Jing Plant Science Institute, School of Life Sciences, Yunnan University, 650091, Kunming, China aut Li Yuzhong Plant Science Institute, School of Life Sciences, Yunnan University, 650091, Kunming, China aut Wang Jianguang Plant Science Institute, School of Life Sciences, Yunnan University, 650091, Kunming, China aut Ni Hongmei Plant Science Institute, School of Life Sciences, Yunnan University, 650091, Kunming, China aut Xia Guangmin School of Life Sciences, Shandong University, 250100, Jinan, China aut Chen Suiyun Plant Science Institute, School of Life Sciences, Yunnan University, 650091, Kunming, China aut Cytotechnology Springer Netherlands 63/6(2011-12-01), 599-607 0920-9069 63:6<599 2011 63 10616 https://doi.org/10.1007/s10616-011-9381-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10616-011-9381-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dai Jinran Plant Science Institute, School of Life Sciences, Yunnan University, 650091, Kunming, China aut NATIONALLICENCE 700 1- Ma Haiyin Plant Science Institute, School of Life Sciences, Yunnan University, 650091, Kunming, China aut NATIONALLICENCE 700 1- Fan Jing Plant Science Institute, School of Life Sciences, Yunnan University, 650091, Kunming, China aut NATIONALLICENCE 700 1- Li Yuzhong Plant Science Institute, School of Life Sciences, Yunnan University, 650091, Kunming, China aut NATIONALLICENCE 700 1- Wang Jianguang Plant Science Institute, School of Life Sciences, Yunnan University, 650091, Kunming, China aut NATIONALLICENCE 700 1- Ni Hongmei Plant Science Institute, School of Life Sciences, Yunnan University, 650091, Kunming, China aut NATIONALLICENCE 700 1- Xia Guangmin School of Life Sciences, Shandong University, 250100, Jinan, China aut NATIONALLICENCE 700 1- Chen Suiyun Plant Science Institute, School of Life Sciences, Yunnan University, 650091, Kunming, China aut NATIONALLICENCE 773 0- Cytotechnology Springer Netherlands 63/6(2011-12-01), 599-607 0920-9069 63:6<599 2011 63 10616 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer