caa a22 4500 445823089 CHVBK 20180317145250.0 cr unu---uuuuu 170323e20110501xx s 000 0 eng 10.1007/s10616-011-9337-x doi (NATIONALLICENCE)springer-10.1007/s10616-011-9337-x In vitro trans-differentiation of human umbilical cord derived hematopoietic stem cells into hepatocyte like cells using combination of growth factors for cell based therapy [Elektronische Daten] [S. Sellamuthu, R. Manikandan, R. Thiagarajan, G. Babu, D. Dinesh, D. Prabhu, C. Arulvasu] The aim of the study was to develop a new strategy for the differentiation of hematopoietic stem cell (HSC) derived from UCB into hepatocyte like cells and also to estimate the effects of combination of fibroblast growth factor 4 (FGF 4) and hepatocyte growth factor (HGF) on hematopoietic stem cell differentiation. HSCs were isolated and purified by magnetic activated cell sorting. HSCs were induced to hepatocyte like cells under a 2-step protocol with combination of growth factors. Reverse transcription polymerase chain reaction was performed to detect multiple genes related to hepatocyte like cells development and function. Hepatocyte like morphology was illustrated by inverted repeat microscope and the secretion of albumin and α- fetoprotein by these cells was confirmed by enzyme linked immunosorbent assay. Hepatocyte like cells was observed at the end of the protocol (days 14). These differentiated cells were observed to show high expression of genes related to hepatocytes (tryptophan 2, 3-dioxygenase [TO], glucose 6-phosphate [G6P], cytokeratin 18 [CK 18], albumin and α- fetoprotein [AFP]). The quantities of albumin and AFP at day 0 were low and upon differentiation the cells were able to produce albumin and AFP at high levels. Our results show a new strategy for differentiation in a short duration, using a combination of growth factors for the differentiation of umbilical cord blood derived HSC into hepatocyte like cells under certain in vitro conditions. After further studies this approach has the potency, for widespread cell replacement therapy for liver diseases. Springer Science+Business Media B.V., 2011 Growth factors nationallicence Hematopoietic stem cell nationallicence Hepatocyte nationallicence Trans-differentiation nationallicence Umbilical cord blood nationallicence Sellamuthu S. Department of Zoology, University of Madras, Guindy Campus, 600 025, Chennai, Tamilnadu, India aut Manikandan R. Department of Animal Health and Management, Alagappa University, 03, Karaikudi, Tamilnadu, India aut Thiagarajan R. Department of Biotechnology, School of Chemical and Biotechnology, Sastra University, 613 401, Thanjavur, Tamilnadu, India aut Babu G. Department of Zoology, University of Madras, Guindy Campus, 600 025, Chennai, Tamilnadu, India aut Dinesh D. Department of Zoology, University of Madras, Guindy Campus, 600 025, Chennai, Tamilnadu, India aut Prabhu D. Department of Zoology, University of Madras, Guindy Campus, 600 025, Chennai, Tamilnadu, India aut Arulvasu C. Department of Zoology, University of Madras, Guindy Campus, 600 025, Chennai, Tamilnadu, India aut Cytotechnology Springer Netherlands 63/3(2011-05-01), 259-268 0920-9069 63:3<259 2011 63 10616 https://doi.org/10.1007/s10616-011-9337-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10616-011-9337-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sellamuthu S. Department of Zoology, University of Madras, Guindy Campus, 600 025, Chennai, Tamilnadu, India aut NATIONALLICENCE 700 1- Manikandan R. Department of Animal Health and Management, Alagappa University, 03, Karaikudi, Tamilnadu, India aut NATIONALLICENCE 700 1- Thiagarajan R. Department of Biotechnology, School of Chemical and Biotechnology, Sastra University, 613 401, Thanjavur, Tamilnadu, India aut NATIONALLICENCE 700 1- Babu G. Department of Zoology, University of Madras, Guindy Campus, 600 025, Chennai, Tamilnadu, India aut NATIONALLICENCE 700 1- Dinesh D. Department of Zoology, University of Madras, Guindy Campus, 600 025, Chennai, Tamilnadu, India aut NATIONALLICENCE 700 1- Prabhu D. Department of Zoology, University of Madras, Guindy Campus, 600 025, Chennai, Tamilnadu, India aut NATIONALLICENCE 700 1- Arulvasu C. Department of Zoology, University of Madras, Guindy Campus, 600 025, Chennai, Tamilnadu, India aut NATIONALLICENCE 773 0- Cytotechnology Springer Netherlands 63/3(2011-05-01), 259-268 0920-9069 63:3<259 2011 63 10616 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer