caa a22 4500 445823097 CHVBK 20180317145250.0 cr unu---uuuuu 170323e20110501xx s 000 0 eng 10.1007/s10616-011-9338-9 doi (NATIONALLICENCE)springer-10.1007/s10616-011-9338-9 A culture system for the live analysis of successive developmental processes and the morphological control of mammalian vertebral cartilage [Elektronische Daten] [Yuichi Aono, Yohei Hirai] The mesoderm-derived segmental somite differentiates into dermomyotome and sclerotome, the latter of which undergoes vertebrogenesis to spinal cartilage and ultimately to vertebral bones. However, analysis and manipulation of the developing mammalian vertebrae in the same embryo has been infeasible because of their placental-dependent embryogenesis. Here, we report a novel culture system of the mouse embryonic tailbud, by which the developmental processes of mammalian vertebral cartilage are traceable and manipulatable in the same sample. The anaplastic segmental somites/sclerotomes in the tailbud of 13 gestational day (g.d.) embryo that are structurally continuous to the vertebral column underwent progressive vertebrogenesis when (1) the ectoderm-derived nascent epidermis was microsurgically removed prior to cultivation, and (2) the sample was incubated at the air-medium interface. After cultivation for 5days, the size and shape of the instructed vertebral cartilage showed features comparable to well-differentiated body vertebra along with the expression of the cartilage marker collagen type II, suggesting that aggressive differentiation of the sclerotomal cell lineage was achieved. In the presence of recombinant bone morphogenic protein (BMP) and Noggin, or adenoviral particles for extracellular epimorphin, dramatic alteration of the vertebral morphology ensued in the explants. Thus, this model system provides an approach to study the detailed molecular mechanisms of mammalian vertebrogenesis and enables pretreatment strategies of precartilagious fragments for improving the efficacy of subsequent transplantation. Springer Science+Business Media B.V., 2011 Vertebrogenesis nationallicence Live analysis nationallicence Cartilage nationallicence Epimorphin nationallicence Organ culture nationallicence Morphogenesis nationallicence Aono Yuichi Department of Bioscience, School of Science and Technology, Kwansei Gakuin University, 2-1 Gakuen, 669-1337, Sanda, Japan aut Hirai Yohei Department of Bioscience, School of Science and Technology, Kwansei Gakuin University, 2-1 Gakuen, 669-1337, Sanda, Japan aut Cytotechnology Springer Netherlands 63/3(2011-05-01), 269-277 0920-9069 63:3<269 2011 63 10616 https://doi.org/10.1007/s10616-011-9338-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10616-011-9338-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Aono Yuichi Department of Bioscience, School of Science and Technology, Kwansei Gakuin University, 2-1 Gakuen, 669-1337, Sanda, Japan aut NATIONALLICENCE 700 1- Hirai Yohei Department of Bioscience, School of Science and Technology, Kwansei Gakuin University, 2-1 Gakuen, 669-1337, Sanda, Japan aut NATIONALLICENCE 773 0- Cytotechnology Springer Netherlands 63/3(2011-05-01), 269-277 0920-9069 63:3<269 2011 63 10616 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer