caa a22 4500 445823127 CHVBK 20180317145250.0 cr unu---uuuuu 170323e20110501xx s 000 0 eng 10.1007/s10616-011-9340-2 doi (NATIONALLICENCE)springer-10.1007/s10616-011-9340-2 A fusion protein composed of receptor binding domain of vascular endothelial growth factor-A and constant region fragment of antibody: angiogenesis antagonistic activity [Elektronische Daten] [Feng-Jen Tseng, Yen-Ku Liu, Yo-Shong Chung, Yu-Ling Lin, Chia-Hung Chen, Wan-Yi Wang, Yu-Cheng Chen, Nu-Man Tsai, Tian-Lu Cheng, Ru-Yu Pan, Tsung-Ming Hu, Ru-Ping Lee, Kuang-Wen Liao] Vascular endothelial growth factor (VEGF) promotes the growth of solid tumor mainly via VEGF receptor-1 and receptor-2, which are expressed preferentially in proliferating endothelial cells. Therefore, a strategy for simultaneous blockage of both VEGF receptors may have a useful therapeutic effect in tumor growth. In this study, we utilized a fusion protein which is composed of receptor binding domain of VEGF-A (RBDV) and the constant region fragment (Fc) of a human immunoglobulin G1 (IgG1), to interfere with the growth of human umbilical vein endothelial cells (HUVECs) via VEGF receptors. The results showed that RBDV-IgG1 Fc was able to bind with both VEGF receptor-1 and receptor-2. In addition, RBDV-IgG1 Fc could decrease VEGF-induced proliferation and tube formation among HUVECs. Moreover, the cytotoxic test showed RBDV-IgG1 Fc could also enhance the cytotoxic activity of human natural killing cells. The data are suggesting that the fusion protein, RBDV-IgG1 Fc, may have potential as an angiogenesis antagonist for future tumor therapy. Springer Science+Business Media B.V., 2011 Vascular endothelial growth factor nationallicence Receptor binding domain of VEGF-A nationallicence Immunoglobulin nationallicence Fusion protein nationallicence Human umbilical vein endothelial cells nationallicence Tseng Feng-Jen Department of Orthopedics, Hualien Armed Forces Hospital, 97144, Hualien, Taiwan, ROC aut Liu Yen-Ku Institute of Molecular Medicine and Bioengineering, National Chiao Tung University, 30050, Hsin-Chu, Taiwan, ROC aut Chung Yo-Shong Department of Biological Science and Technology, National Chiao Tung University, 30050, Hsin-Chu, Taiwan, ROC aut Lin Yu-Ling Institute of Molecular Medicine and Bioengineering, National Chiao Tung University, 30050, Hsin-Chu, Taiwan, ROC aut Chen Chia-Hung Institute of Molecular Medicine and Bioengineering, National Chiao Tung University, 30050, Hsin-Chu, Taiwan, ROC aut Wang Wan-Yi Department of Biological Science and Technology, National Chiao Tung University, 30050, Hsin-Chu, Taiwan, ROC aut Chen Yu-Cheng Institute of Molecular Medicine and Bioengineering, National Chiao Tung University, 30050, Hsin-Chu, Taiwan, ROC aut Tsai Nu-Man School of Medical Laboratory and Biotechnology, Chung Shan Medical University, 40203, Taichung, Taiwan, ROC aut Cheng Tian-Lu Department of Biomedical Science and Environmental Biology, Kaohsiung Medical University, 807, Kaohsiung, Taiwan, ROC aut Pan Ru-Yu Graduate Institute of Medical Science, National Defense Medical Center, 114, Taipei, Taiwan, ROC aut Hu Tsung-Ming Department of Psychiatry, Yuli Veterans Hospital, 98142, Hualien, Taiwan, ROC aut Lee Ru-Ping Institute of Nursing and Medical Sciences and Department of Physiology, Tzu Chi University, 97004, Hualien, Taiwan, ROC aut Liao Kuang-Wen Department of Biological Science and Technology, National Chiao Tung University, 30050, Hsin-Chu, Taiwan, ROC aut Cytotechnology Springer Netherlands 63/3(2011-05-01), 285-293 0920-9069 63:3<285 2011 63 10616 https://doi.org/10.1007/s10616-011-9340-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10616-011-9340-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tseng Feng-Jen Department of Orthopedics, Hualien Armed Forces Hospital, 97144, Hualien, Taiwan, ROC aut NATIONALLICENCE 700 1- Liu Yen-Ku Institute of Molecular Medicine and Bioengineering, National Chiao Tung University, 30050, Hsin-Chu, Taiwan, ROC aut NATIONALLICENCE 700 1- Chung Yo-Shong Department of Biological Science and Technology, National Chiao Tung University, 30050, Hsin-Chu, Taiwan, ROC aut NATIONALLICENCE 700 1- Lin Yu-Ling Institute of Molecular Medicine and Bioengineering, National Chiao Tung University, 30050, Hsin-Chu, Taiwan, ROC aut NATIONALLICENCE 700 1- Chen Chia-Hung Institute of Molecular Medicine and Bioengineering, National Chiao Tung University, 30050, Hsin-Chu, Taiwan, ROC aut NATIONALLICENCE 700 1- Wang Wan-Yi Department of Biological Science and Technology, National Chiao Tung University, 30050, Hsin-Chu, Taiwan, ROC aut NATIONALLICENCE 700 1- Chen Yu-Cheng Institute of Molecular Medicine and Bioengineering, National Chiao Tung University, 30050, Hsin-Chu, Taiwan, ROC aut NATIONALLICENCE 700 1- Tsai Nu-Man School of Medical Laboratory and Biotechnology, Chung Shan Medical University, 40203, Taichung, Taiwan, ROC aut NATIONALLICENCE 700 1- Cheng Tian-Lu Department of Biomedical Science and Environmental Biology, Kaohsiung Medical University, 807, Kaohsiung, Taiwan, ROC aut NATIONALLICENCE 700 1- Pan Ru-Yu Graduate Institute of Medical Science, National Defense Medical Center, 114, Taipei, Taiwan, ROC aut NATIONALLICENCE 700 1- Hu Tsung-Ming Department of Psychiatry, Yuli Veterans Hospital, 98142, Hualien, Taiwan, ROC aut NATIONALLICENCE 700 1- Lee Ru-Ping Institute of Nursing and Medical Sciences and Department of Physiology, Tzu Chi University, 97004, Hualien, Taiwan, ROC aut NATIONALLICENCE 700 1- Liao Kuang-Wen Department of Biological Science and Technology, National Chiao Tung University, 30050, Hsin-Chu, Taiwan, ROC aut NATIONALLICENCE 773 0- Cytotechnology Springer Netherlands 63/3(2011-05-01), 285-293 0920-9069 63:3<285 2011 63 10616 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer