caa a22 4500 445823178 CHVBK 20180317145250.0 cr unu---uuuuu 170323e20110501xx s 000 0 eng 10.1007/s10616-011-9349-6 doi (NATIONALLICENCE)springer-10.1007/s10616-011-9349-6 Orally administered Bifidobacterium triggers immune responses following capture by CD11c+ cells in Peyer's patches and cecal patches [Elektronische Daten] [Yasuhiro Hiramatsu, Akira Hosono, Takuma Konno, Yusuke Nakanishi, Masamichi Muto, Akari Suyama, Satoshi Hachimura, Ryuichiro Sato, Kyoko Takahashi, Shuichi Kaminogawa] We have investigated the immunomodulatory mechanisms of Bifidobacterium pseudocatenulatum JCM7041 (Bp) as model of probiotics following oral administration to mice. This study was conducted with the aim of clarifying the mechanism of immunomodulation induced by oral administration of probiotic bacteria through elucidation of the detailed mechanism of transfer of orally administered bacterial cells within the body and the interaction between bacterial cells and cells of the immune tissues. We observed the localization of Bp in mice following oral administration, showing that Bp was surrounded by CD11c+ cells in Peyer's patches (PP) and cecal patches (CP). These results indicated that Bp might induce CD11c+ cell-mediated immune responses directly. Furthermore, IL-10 and IL-12p40 production by Thy1.2− cells, including CD11c+ cells, increased significantly. Production of IL-10 and IL-12p40 by bone marrow-derived dendritic cells (BMDC) was significantly increased by Bp stimulation. These results suggest that oral administration of Bp induces immune responses directly following capture by CD11c+ dendritic cells (DCs). Subsequently, we observed oral administration of Bp for 1week induced IgA and IgA-associated cytokine production by CP and PP cells, suggesting that Bp induced DC-mediated immune responses on CP as well as PP. Springer Science+Business Media B.V., 2011 Probiotics nationallicence Bifidobacterium nationallicence Dendritic cells nationallicence Bp : Bifidobacterium pseudocatenulatum JCM7041 nationallicence BMDC : Bone marrow-derived dendritic cells nationallicence BIM : Bifidobacterium immunomodulator nationallicence Hiramatsu Yasuhiro Department of Food Bioscience and Biotechnology, College of Bioresource Sciences, Nihon University, 1866, Kameino, 252-0880, Fujisawa-shi, Kanagawa, Japan aut Hosono Akira Department of Food Bioscience and Biotechnology, College of Bioresource Sciences, Nihon University, 1866, Kameino, 252-0880, Fujisawa-shi, Kanagawa, Japan aut Konno Takuma Department of Food Bioscience and Biotechnology, College of Bioresource Sciences, Nihon University, 1866, Kameino, 252-0880, Fujisawa-shi, Kanagawa, Japan aut Nakanishi Yusuke Department of Food Bioscience and Biotechnology, College of Bioresource Sciences, Nihon University, 1866, Kameino, 252-0880, Fujisawa-shi, Kanagawa, Japan aut Muto Masamichi Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1, Yayoi, Bunkyo-ku, 113-8657, Tokyo, Japan aut Suyama Akari Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1, Yayoi, Bunkyo-ku, 113-8657, Tokyo, Japan aut Hachimura Satoshi Research Center for Food Safety, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1, Yayoi, Bunkyo-ku, 113-8657, Tokyo, Japan aut Sato Ryuichiro Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1, Yayoi, Bunkyo-ku, 113-8657, Tokyo, Japan aut Takahashi Kyoko Department of Food Bioscience and Biotechnology, College of Bioresource Sciences, Nihon University, 1866, Kameino, 252-0880, Fujisawa-shi, Kanagawa, Japan aut Kaminogawa Shuichi Department of Food Bioscience and Biotechnology, College of Bioresource Sciences, Nihon University, 1866, Kameino, 252-0880, Fujisawa-shi, Kanagawa, Japan aut Cytotechnology Springer Netherlands 63/3(2011-05-01), 307-317 0920-9069 63:3<307 2011 63 10616 https://doi.org/10.1007/s10616-011-9349-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10616-011-9349-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hiramatsu Yasuhiro Department of Food Bioscience and Biotechnology, College of Bioresource Sciences, Nihon University, 1866, Kameino, 252-0880, Fujisawa-shi, Kanagawa, Japan aut NATIONALLICENCE 700 1- Hosono Akira Department of Food Bioscience and Biotechnology, College of Bioresource Sciences, Nihon University, 1866, Kameino, 252-0880, Fujisawa-shi, Kanagawa, Japan aut NATIONALLICENCE 700 1- Konno Takuma Department of Food Bioscience and Biotechnology, College of Bioresource Sciences, Nihon University, 1866, Kameino, 252-0880, Fujisawa-shi, Kanagawa, Japan aut NATIONALLICENCE 700 1- Nakanishi Yusuke Department of Food Bioscience and Biotechnology, College of Bioresource Sciences, Nihon University, 1866, Kameino, 252-0880, Fujisawa-shi, Kanagawa, Japan aut NATIONALLICENCE 700 1- Muto Masamichi Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1, Yayoi, Bunkyo-ku, 113-8657, Tokyo, Japan aut NATIONALLICENCE 700 1- Suyama Akari Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1, Yayoi, Bunkyo-ku, 113-8657, Tokyo, Japan aut NATIONALLICENCE 700 1- Hachimura Satoshi Research Center for Food Safety, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1, Yayoi, Bunkyo-ku, 113-8657, Tokyo, Japan aut NATIONALLICENCE 700 1- Sato Ryuichiro Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1, Yayoi, Bunkyo-ku, 113-8657, Tokyo, Japan aut NATIONALLICENCE 700 1- Takahashi Kyoko Department of Food Bioscience and Biotechnology, College of Bioresource Sciences, Nihon University, 1866, Kameino, 252-0880, Fujisawa-shi, Kanagawa, Japan aut NATIONALLICENCE 700 1- Kaminogawa Shuichi Department of Food Bioscience and Biotechnology, College of Bioresource Sciences, Nihon University, 1866, Kameino, 252-0880, Fujisawa-shi, Kanagawa, Japan aut NATIONALLICENCE 773 0- Cytotechnology Springer Netherlands 63/3(2011-05-01), 307-317 0920-9069 63:3<307 2011 63 10616 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer