caa a22 4500 445823186 CHVBK 20180317145250.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s10616-011-9356-7 doi (NATIONALLICENCE)springer-10.1007/s10616-011-9356-7 Characterization of in vitro cultured myoblasts isolated from duck ( Anas platyrhynchos ) embryo [Elektronische Daten] [He-he Liu, Liang Li, Xi Chen, Wei Cao, Rong-ping Zhang, Hai-yue Yu, Feng Xu, Hua He, Ji-wen Wang] Myoblasts isolated from duck embryonic muscle were purified and in vitro cultured. External characteristics were observed by using the immunofluorescence technique, and growth curve of duck embryonic myoblasts was established after measuring with the MTT method. Moreover, mRNA expression of three marker genes, the Desmin, the muscle creatine kinase (Mck) and the troponin C (Tnnc), which could reflect the development status of myofibers, were detected each 24h for cultured cells by using the qPCR technique. Results showed that the in vitro cultured duck myoblasts went through a series of developmental stages, including the proliferation of myoblasts, the differentiation of multi-nuclei myotubes, and the formation of myofiber. The cultured duck embryonic myoblasts entered into a logarithmic stage approximately on the fourth day after seeding. Accompanying with its progressive growth before entering into the logarithmic phase, the myoblasts also showed some differentiation phenomena, reflected by a low expression level of Desmin and high expression level of the Mck and Tnnc genes. During the rapid growth of the logarithmic phase, there was a high expression of the Desmin gene, and a low expression level of the Mck gene and the Tnnc gene in the cultured myoblasts. The expression profiles of the three marker genes for muscle development could be used for distinguishing the different developmental stages of in vitro cultured myoblasts at the molecular level, which would be more accurate and more feasible than observing the external characteristics of the cultured cells. Springer Science+Business Media B.V., 2011 Gene expression nationallicence Embryonic myoblasts nationallicence Cell growth nationallicence Liu He-he Key Lab of Animal Genetic Resources, College of Animal Science and Technology, Sichuan Agricultural University, 625014, Ya'an, Sichuan, People's Republic of China aut Li Liang Key Lab of Animal Genetic Resources, College of Animal Science and Technology, Sichuan Agricultural University, 625014, Ya'an, Sichuan, People's Republic of China aut Chen Xi Key Lab of Animal Genetic Resources, College of Animal Science and Technology, Sichuan Agricultural University, 625014, Ya'an, Sichuan, People's Republic of China aut Cao Wei Key Lab of Animal Genetic Resources, College of Animal Science and Technology, Sichuan Agricultural University, 625014, Ya'an, Sichuan, People's Republic of China aut Zhang Rong-ping Key Lab of Animal Genetic Resources, College of Animal Science and Technology, Sichuan Agricultural University, 625014, Ya'an, Sichuan, People's Republic of China aut Yu Hai-yue Key Lab of Animal Genetic Resources, College of Animal Science and Technology, Sichuan Agricultural University, 625014, Ya'an, Sichuan, People's Republic of China aut Xu Feng Key Lab of Animal Genetic Resources, College of Animal Science and Technology, Sichuan Agricultural University, 625014, Ya'an, Sichuan, People's Republic of China aut He Hua Key Lab of Animal Genetic Resources, College of Animal Science and Technology, Sichuan Agricultural University, 625014, Ya'an, Sichuan, People's Republic of China aut Wang Ji-wen Key Lab of Animal Genetic Resources, College of Animal Science and Technology, Sichuan Agricultural University, 625014, Ya'an, Sichuan, People's Republic of China aut Cytotechnology Springer Netherlands 63/4(2011-08-01), 399-406 0920-9069 63:4<399 2011 63 10616 https://doi.org/10.1007/s10616-011-9356-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10616-011-9356-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liu He-he Key Lab of Animal Genetic Resources, College of Animal Science and Technology, Sichuan Agricultural University, 625014, Ya'an, Sichuan, People's Republic of China aut NATIONALLICENCE 700 1- Li Liang Key Lab of Animal Genetic Resources, College of Animal Science and Technology, Sichuan Agricultural University, 625014, Ya'an, Sichuan, People's Republic of China aut NATIONALLICENCE 700 1- Chen Xi Key Lab of Animal Genetic Resources, College of Animal Science and Technology, Sichuan Agricultural University, 625014, Ya'an, Sichuan, People's Republic of China aut NATIONALLICENCE 700 1- Cao Wei Key Lab of Animal Genetic Resources, College of Animal Science and Technology, Sichuan Agricultural University, 625014, Ya'an, Sichuan, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Rong-ping Key Lab of Animal Genetic Resources, College of Animal Science and Technology, Sichuan Agricultural University, 625014, Ya'an, Sichuan, People's Republic of China aut NATIONALLICENCE 700 1- Yu Hai-yue Key Lab of Animal Genetic Resources, College of Animal Science and Technology, Sichuan Agricultural University, 625014, Ya'an, Sichuan, People's Republic of China aut NATIONALLICENCE 700 1- Xu Feng Key Lab of Animal Genetic Resources, College of Animal Science and Technology, Sichuan Agricultural University, 625014, Ya'an, Sichuan, People's Republic of China aut NATIONALLICENCE 700 1- He Hua Key Lab of Animal Genetic Resources, College of Animal Science and Technology, Sichuan Agricultural University, 625014, Ya'an, Sichuan, People's Republic of China aut NATIONALLICENCE 700 1- Wang Ji-wen Key Lab of Animal Genetic Resources, College of Animal Science and Technology, Sichuan Agricultural University, 625014, Ya'an, Sichuan, People's Republic of China aut NATIONALLICENCE 773 0- Cytotechnology Springer Netherlands 63/4(2011-08-01), 399-406 0920-9069 63:4<399 2011 63 10616 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer